Introduction: In recent years, oxidative stress has been postulated to be an important factor in pathogenesis and development of diseases. Reactive Oxygen Species (ROS) are well known for being both beneficial and deleterious and have long been known to be a component of the killing response of immune cells to microbial invasion. Measuring the free radical activity in serum samples is the best way to investigate the association between oxidative stress and infectious diseases. The aim of this study is to determine the association between alphaherpesvirus infection and serum reactive oxygen metabolites (ROM) in Italian Mediterranean buffaloes (Bubalus bubalis). Materials and Methods: The presence of antibodies against bovine herpesvirus type 1 (BoHV-1) or bubaline herpesvirus type 1 (BuHV-1) was investigated by enzyme-linked immunosorbent assay (anti- gB/gE blocking ELISA) (Idexx). We carried out the derivatives of ROM (d-ROM) and the antioxidant activity (anti-ROM) tests by spectrophotometry in sera of BoHV-1 (A) or BuHV-1 (B) infected animals compared to seronegative animals (C). Results: The concentrations of d-ROM in the three groups, expressed in Carratelli Units (U-CARR) were, respectively, 64.6 ± 11.6, 85.0 ± 10.7 and 56.2 ± 10.5. Although the serum d-ROM levels in group A and B were higher than that in group C, only the group B compared with the control group C was significant at p < 0.05. At anti-ROM test, the three groups of animals showed values respectively of: 287.5 ± 66.1, 318 ± 64.3 and 276 ± 53.0. The results of group A and B compared to that of control group C were not significant. The oxidative stress index (OSI), calculated as d-ROM/anti-ROM x 100, showed significant differences between the groups B (26) and C (20) whereas no significant difference was detected between the groups A (22) and C (20). Discussion and Conclusions: Our data indicate that in alphaherpesvirus infected buffaloes, in particular those in BuHV-1 infected, the d-ROM and the OSI values were significantly increased. These results suggest that the presence of BuHV-1, the virus species-specific, seems to induce a worsening balance in ROM levels, whereas that of BoHV-1 has no substantial effects. Future studies are needed to assess the role of oxidative stress in Bubalus bubalis infected by alphaherpesvirus.
Relations between serum reactive oxygen metabolites and alphaherpesvirus infection in Italian Mediterranean buffaloes - Bubalus bubalis / Marullo, Annarosaria; Tafuri, Simona; Ciani, Francesca; Della Morte, Rossella; Nocera, Francesca Paola; Fiorito, Filomena; Iovane, Giuseppe; De Martino, Luisa. - ELETTRONICO. - (2015). (Intervento presentato al convegno 43th National Congress of the Italian Society Microbiology (S.I.M.) tenutosi a Napoli (Italy) nel 27 - 30 September 2015).
Relations between serum reactive oxygen metabolites and alphaherpesvirus infection in Italian Mediterranean buffaloes - Bubalus bubalis
MARULLO, ANNAROSARIA;
2015
Abstract
Introduction: In recent years, oxidative stress has been postulated to be an important factor in pathogenesis and development of diseases. Reactive Oxygen Species (ROS) are well known for being both beneficial and deleterious and have long been known to be a component of the killing response of immune cells to microbial invasion. Measuring the free radical activity in serum samples is the best way to investigate the association between oxidative stress and infectious diseases. The aim of this study is to determine the association between alphaherpesvirus infection and serum reactive oxygen metabolites (ROM) in Italian Mediterranean buffaloes (Bubalus bubalis). Materials and Methods: The presence of antibodies against bovine herpesvirus type 1 (BoHV-1) or bubaline herpesvirus type 1 (BuHV-1) was investigated by enzyme-linked immunosorbent assay (anti- gB/gE blocking ELISA) (Idexx). We carried out the derivatives of ROM (d-ROM) and the antioxidant activity (anti-ROM) tests by spectrophotometry in sera of BoHV-1 (A) or BuHV-1 (B) infected animals compared to seronegative animals (C). Results: The concentrations of d-ROM in the three groups, expressed in Carratelli Units (U-CARR) were, respectively, 64.6 ± 11.6, 85.0 ± 10.7 and 56.2 ± 10.5. Although the serum d-ROM levels in group A and B were higher than that in group C, only the group B compared with the control group C was significant at p < 0.05. At anti-ROM test, the three groups of animals showed values respectively of: 287.5 ± 66.1, 318 ± 64.3 and 276 ± 53.0. The results of group A and B compared to that of control group C were not significant. The oxidative stress index (OSI), calculated as d-ROM/anti-ROM x 100, showed significant differences between the groups B (26) and C (20) whereas no significant difference was detected between the groups A (22) and C (20). Discussion and Conclusions: Our data indicate that in alphaherpesvirus infected buffaloes, in particular those in BuHV-1 infected, the d-ROM and the OSI values were significantly increased. These results suggest that the presence of BuHV-1, the virus species-specific, seems to induce a worsening balance in ROM levels, whereas that of BoHV-1 has no substantial effects. Future studies are needed to assess the role of oxidative stress in Bubalus bubalis infected by alphaherpesvirus.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
