The quali-quantitative characterization of the oral microbiota is crucial for an exhaustive knowledge of the oral ecology and the modifcations of the microbial composition that occur during periodontal pathologies. In this study, we designed and validated a new phylogenetic DNA-microarray (OralArray) to quickly and reliably characterize the most representative bacterial groups that colonize the oral cavity. The OralArray is based on the Ligation Detection Reaction technology associated to Universal Arrays (LDR-UA), and includes 22 probe sets targeted to bacteria belonging to the phyla Firmicutes, Proteobacteria, Actinobacteria, Bacteroidetes, Fusobacteria, and Spirochaete. The tool is characterized by high specifcity, sensitivity and reproducibility. The OralArray was successfully tested and validated on diferent oral samples (saliva, lingual plaque, supragingival plaque, and healing cap) collected from 10 healthy subjects. For each specimen, a microbial signature was obtained, and our results established the presence of an oral microbial profle specifc for each subject. Moreover, the tool was applied to evaluate the efcacy of a disinfectant treatment on the healing caps before their usage. The OralArray is, thus, suitable to study the microbiota associated with various oral sites and to monitor changes arising from therapeutic treatments.

Parolin, C.E., Giordani, B., NAHUI PALOMINO, R.A., Biagi, E., Severgnini, M., Consolandi, C., et al. (2017). Design and validation of a DNA-microarray for phylogenetic analysis of bacterial communities in different oral samples and dental implants. SCIENTIFIC REPORTS, 7(1), 1-12 [10.1038/s41598-017-06743-6].

Design and validation of a DNA-microarray for phylogenetic analysis of bacterial communities in different oral samples and dental implants

PAROLIN, CAROLA ELEONORA;GIORDANI, BARBARA;NAHUI PALOMINO, ROGERS ALBERTO;BIAGI, ELENA;VITALI, BEATRICE
2017

Abstract

The quali-quantitative characterization of the oral microbiota is crucial for an exhaustive knowledge of the oral ecology and the modifcations of the microbial composition that occur during periodontal pathologies. In this study, we designed and validated a new phylogenetic DNA-microarray (OralArray) to quickly and reliably characterize the most representative bacterial groups that colonize the oral cavity. The OralArray is based on the Ligation Detection Reaction technology associated to Universal Arrays (LDR-UA), and includes 22 probe sets targeted to bacteria belonging to the phyla Firmicutes, Proteobacteria, Actinobacteria, Bacteroidetes, Fusobacteria, and Spirochaete. The tool is characterized by high specifcity, sensitivity and reproducibility. The OralArray was successfully tested and validated on diferent oral samples (saliva, lingual plaque, supragingival plaque, and healing cap) collected from 10 healthy subjects. For each specimen, a microbial signature was obtained, and our results established the presence of an oral microbial profle specifc for each subject. Moreover, the tool was applied to evaluate the efcacy of a disinfectant treatment on the healing caps before their usage. The OralArray is, thus, suitable to study the microbiota associated with various oral sites and to monitor changes arising from therapeutic treatments.
2017
Parolin, C.E., Giordani, B., NAHUI PALOMINO, R.A., Biagi, E., Severgnini, M., Consolandi, C., et al. (2017). Design and validation of a DNA-microarray for phylogenetic analysis of bacterial communities in different oral samples and dental implants. SCIENTIFIC REPORTS, 7(1), 1-12 [10.1038/s41598-017-06743-6].
Parolin, CAROLA ELEONORA; Giordani, Barbara; NAHUI PALOMINO, ROGERS ALBERTO; Biagi, Elena; Severgnini, M.; Consolandi, C.; Caredda, G.; Storelli, S.; ...espandi
File in questo prodotto:
File Dimensione Formato  
ParolinC_2017.pdf

accesso aperto

Tipo: Versione (PDF) editoriale
Licenza: Licenza per Accesso Aperto. Creative Commons Attribuzione (CCBY)
Dimensione 1.67 MB
Formato Adobe PDF
1.67 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/605211
Citazioni
  • ???jsp.display-item.citation.pmc??? 3
  • Scopus 16
  • ???jsp.display-item.citation.isi??? 9
social impact