We successfully combined ultrastructure and chloroplast DNA analysis for single specimens of Concentricystis isolated from Holocene sediment by a palynology method based on filtration, progressive sieving and percoll-gradient sedimentation to concentrate fossil cells. We compared our method with the traditional harsh chemical treatment commonly used to isolate fossil pollen and spores. With our method, the cytoplasm of Concentricystis was sufficiently preserved to distinguish several cell structures by light and electron microscopy. DNA analysis of Concentricystis involved PCR amplification using specific primers for a spacer region of the chloroplast genome. Significant homologies were found with the Angiosperm chloroplast genome by BLAST DNA search for PCR product sequences.
MILANESI C, VIGNANI R, CIAMPOLINI F, FALERI C, A. MORONI, ARRIGHI S, et al. (2006). Ultrastructure and DNA sequence analysis of single Concentricystis cell from Alta Val Tiberina Holocene sediment. JOURNAL OF ARCHAEOLOGICAL SCIENCE, 33(8), 1081-1087 [10.1016/j.jas.2005.11.014].
Ultrastructure and DNA sequence analysis of single Concentricystis cell from Alta Val Tiberina Holocene sediment.
ARRIGHI, SIMONA;
2006
Abstract
We successfully combined ultrastructure and chloroplast DNA analysis for single specimens of Concentricystis isolated from Holocene sediment by a palynology method based on filtration, progressive sieving and percoll-gradient sedimentation to concentrate fossil cells. We compared our method with the traditional harsh chemical treatment commonly used to isolate fossil pollen and spores. With our method, the cytoplasm of Concentricystis was sufficiently preserved to distinguish several cell structures by light and electron microscopy. DNA analysis of Concentricystis involved PCR amplification using specific primers for a spacer region of the chloroplast genome. Significant homologies were found with the Angiosperm chloroplast genome by BLAST DNA search for PCR product sequences.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
