The characterization of root system architecture (RSA) is receiving increasing attention because of its potential implications in plant breeding. Nevertheless, the intrinsic difficulty of roots phenotyping remains a major challenge towared against the comprehensive understanding of roots development and its impact on shoot growth and nutrients/water uptake. In this study, we present the characterization of RSA in 183 elite durum wheat (Triticum turgidum L. var. durum Desf.) from the seedling stage up to the 6th-7th leaf appearance (late tillering stage). The experiment was performed by mean of the phenotyping platform GROWSCREEN-Rhizo at the Institut für Bio-und Geowissenschaften Pflanzenwissenschaften (IBG-2;Jülich, Germany) This platform includes 72 soil-filled custom-made rhizotrons positioned at a 45 degree angle in each of which it is possible to grow up to four plants. The automated system is suitable for quantitative measurement of root architecture parameters in 2D with typical throughput of 72 rhizotrons/day, allowing for dynamic characterization of root system development. For each plant within the experimental design (four per genotype), the following RSA traits were collected: seminal root length, nodal root length, root system convex hull and root system depth distribution (twice per week). Measurements of leaf area and tiller number were performed twice per week and SPAD measurements were collected twice along the experiment. Root dry biomass and shoot fresh and dry biomass were collected at the end of the experiment. The fingerprinting was carried out with the Illumina Infinium 90K SNP assay. Data analysis is ngoing. Association mapping will allow us to identify chromosomal regions associated with root system development from both a quantitative and qualitative standpoint as well as its relation to shoot growth and tillering in a phenological stage just preceeding the intensive nutrient and water uptake at stem elongation.
ASSOCIATION MAPPING OF ROOT SYSTEM ARCHITECTURE AND SHOOT DEVELOPMENT IN DURUM WHEAT
SCIARA, GIUSEPPE;SALVI, SILVIO;MACCAFERRI, MARCO;TUBEROSA, ROBERTO
2016
Abstract
The characterization of root system architecture (RSA) is receiving increasing attention because of its potential implications in plant breeding. Nevertheless, the intrinsic difficulty of roots phenotyping remains a major challenge towared against the comprehensive understanding of roots development and its impact on shoot growth and nutrients/water uptake. In this study, we present the characterization of RSA in 183 elite durum wheat (Triticum turgidum L. var. durum Desf.) from the seedling stage up to the 6th-7th leaf appearance (late tillering stage). The experiment was performed by mean of the phenotyping platform GROWSCREEN-Rhizo at the Institut für Bio-und Geowissenschaften Pflanzenwissenschaften (IBG-2;Jülich, Germany) This platform includes 72 soil-filled custom-made rhizotrons positioned at a 45 degree angle in each of which it is possible to grow up to four plants. The automated system is suitable for quantitative measurement of root architecture parameters in 2D with typical throughput of 72 rhizotrons/day, allowing for dynamic characterization of root system development. For each plant within the experimental design (four per genotype), the following RSA traits were collected: seminal root length, nodal root length, root system convex hull and root system depth distribution (twice per week). Measurements of leaf area and tiller number were performed twice per week and SPAD measurements were collected twice along the experiment. Root dry biomass and shoot fresh and dry biomass were collected at the end of the experiment. The fingerprinting was carried out with the Illumina Infinium 90K SNP assay. Data analysis is ngoing. Association mapping will allow us to identify chromosomal regions associated with root system development from both a quantitative and qualitative standpoint as well as its relation to shoot growth and tillering in a phenological stage just preceeding the intensive nutrient and water uptake at stem elongation.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
