In this work we present the first results on a 'label-free' DNA analysis system based on hydrogenated amorphous silicon (a-Si:H) thin film sensors. The detection mechanism is based on absorbance measurements performed on DNA molecules in a buffer solution. The experiments made with single-stranded 30-mer DNA at different concentrations showed an excellent linearity over three orders of magnitude. The extrapolated minimum quantity (expressed as concentration - optical path product) detectable by our set-up was 2 nmol×cm, limited by short term variations of the UV source intensity. Furthermore, relying on the hypo-chromic effect it is possible to distinguish between single- and double-stranded DNA chains. This was observed performing DNA hybridization experiment on a functionalized glass substrate.

Label-free DNA analysis using a-Si:H UV sensors

GUIDUCCI, CARLOTTA;RICCO', BRUNO
2005

Abstract

In this work we present the first results on a 'label-free' DNA analysis system based on hydrogenated amorphous silicon (a-Si:H) thin film sensors. The detection mechanism is based on absorbance measurements performed on DNA molecules in a buffer solution. The experiments made with single-stranded 30-mer DNA at different concentrations showed an excellent linearity over three orders of magnitude. The extrapolated minimum quantity (expressed as concentration - optical path product) detectable by our set-up was 2 nmol×cm, limited by short term variations of the UV source intensity. Furthermore, relying on the hypo-chromic effect it is possible to distinguish between single- and double-stranded DNA chains. This was observed performing DNA hybridization experiment on a functionalized glass substrate.
Proceedings of ICST 2005, International Conference on Sensing Technology (IEEE)
254
256
D. Caputo; G. de Cesare; A. Nascetti; C. Guiducci; B. Riccò
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/59896
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