PB12. OVERCOMING BIOSAMPLING ISSUES IN SPORT DRUG TESTING: ANABOLIC STEROIDS IN DRIED URINES Michele PROTTI,1 Maria Carmen CATAPANO,1 Angelo E. SBERNA,2 Stefano GIROTTI,1 James RUDGE,3 and Laura MERCOLINI1 1Department of Pharmacy and Biotechnology (FaBiT), University of Bologna, Via Belmeloro 6, 40126 Bologna, Italy 2 Sport Medicine, Enna Local Health Unit, Viale A. Diaz 7, 94100 Enna, Italy 3 Neoteryx LLC, 421 Amapola Avenue, 90501 Torrance, CA, USA Some crucial points are still under evaluation in the field of sampling and analysis for sport drug testing, including some limitations related to the biological fluid of choice: urine. Dried matrix (DM) collection is a very promising tool for improving anti-doping analysis, addressing those issues related to frequent and impromptu sampling, detection of substance concentrations, enhanced compound stability and need for rapid and high-throughput protocols. Ongoing innovations have demonstrated DM reliability, which is now directly applicable with the coupling to a wealth of microsampling techniques. This research involves in particular the use of dried urine spots (DUS) and volumetric absorptive microsampling (VAMS) on urines, for the collection of accurate volumes, followed by the LC-MS analysis of the main classes of the substances included in the World Anti-Doping Agency (WADA) prohibited list [1], with particular focus on compounds belonging to the class of anabolic steroids (testosterone, epitestosterone, dihydrotestosterone, androstenedione, methandrostenolone, mesterolone, nandrolone, norethandrolone and danazol. The goal of this project is to establish and validate simple but reliable protocols for the collection of urine microvolumes, unlikely to be tampered but transportable and, above all, storable with no precautions, to perform screening tests and targeted analysis according to the International Standard for Testing and Investigations (ISTI) and the International Standard for Laboratories (ISL), as part of the World Anti-Doping Code. These protocols would substantially reduce overall analysis costs, allowing their application not only to elite athletes, but also to amateurs in local laboratories. [1] “The World Anti-Doping Code: the 2016 Prohibited List”. World Anti-Doping Agency (WADA)
Overcoming biosampling issues in sport drug testing: anabolic steroids in dried urines / Protti, M.; Catapano, M.C.; Sberna, A.E.; Girotti, S.; Rudge, J.; Mercolini, L.. - STAMPA. - 1:(2016), pp. 49-49. (Intervento presentato al convegno International Conference “CHIMIA 2016” tenutosi a Constanta, Romania nel 26-28 May 2016).
Overcoming biosampling issues in sport drug testing: anabolic steroids in dried urines
PROTTI, MICHELE;CATAPANO, MARIA CARMEN;GIROTTI, STEFANO;MERCOLINI, LAURA
2016
Abstract
PB12. OVERCOMING BIOSAMPLING ISSUES IN SPORT DRUG TESTING: ANABOLIC STEROIDS IN DRIED URINES Michele PROTTI,1 Maria Carmen CATAPANO,1 Angelo E. SBERNA,2 Stefano GIROTTI,1 James RUDGE,3 and Laura MERCOLINI1 1Department of Pharmacy and Biotechnology (FaBiT), University of Bologna, Via Belmeloro 6, 40126 Bologna, Italy 2 Sport Medicine, Enna Local Health Unit, Viale A. Diaz 7, 94100 Enna, Italy 3 Neoteryx LLC, 421 Amapola Avenue, 90501 Torrance, CA, USA Some crucial points are still under evaluation in the field of sampling and analysis for sport drug testing, including some limitations related to the biological fluid of choice: urine. Dried matrix (DM) collection is a very promising tool for improving anti-doping analysis, addressing those issues related to frequent and impromptu sampling, detection of substance concentrations, enhanced compound stability and need for rapid and high-throughput protocols. Ongoing innovations have demonstrated DM reliability, which is now directly applicable with the coupling to a wealth of microsampling techniques. This research involves in particular the use of dried urine spots (DUS) and volumetric absorptive microsampling (VAMS) on urines, for the collection of accurate volumes, followed by the LC-MS analysis of the main classes of the substances included in the World Anti-Doping Agency (WADA) prohibited list [1], with particular focus on compounds belonging to the class of anabolic steroids (testosterone, epitestosterone, dihydrotestosterone, androstenedione, methandrostenolone, mesterolone, nandrolone, norethandrolone and danazol. The goal of this project is to establish and validate simple but reliable protocols for the collection of urine microvolumes, unlikely to be tampered but transportable and, above all, storable with no precautions, to perform screening tests and targeted analysis according to the International Standard for Testing and Investigations (ISTI) and the International Standard for Laboratories (ISL), as part of the World Anti-Doping Code. These protocols would substantially reduce overall analysis costs, allowing their application not only to elite athletes, but also to amateurs in local laboratories. [1] “The World Anti-Doping Code: the 2016 Prohibited List”. World Anti-Doping Agency (WADA)I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
