OB1. DEVELOPMENT OF A FAST CHEMILUMINESCENT METHOD FOR TESTING AIR POLLUTION Emanuele MORGANTI,1 Miguel AMORIM,2 Luca BOLELLI,1 Elida Nora FERRI,1 Laura TOSITTI,3 Erika BRATTICH,3 Stefano GIROTTI1 1Department of Pharmacy and Biotechnology FaBiT, Via San Donato 15, Alma Mater Studiorum - Università di Bologna, Bologna,40127, Italy 2Faculdade de Farmácia da Universidade de Lisboa, Avenida Prof. Gama Pinto Lisbon, 1649-003, Portugal 3Chemistry Department "Giacomo Ciamician" Via Selmi 2, Alma Mater Studiorum - Università di Bologna, Bologna, 40127, Italy Several epidemiological studies have evidenced the harmful effect of atmospheric particulate matter [1, 2]. The presence of certain substances, such as metals, WSOC (Water Soluble Organic Carbon), sulfur dioxide (SO2), nitrogen dioxide (NO2) and their highly oxidizing radicals SOx and NOx is strongly related to this toxicity [3]. In order to measure the concentration of oxidizing radicals in the air particulate, a chemiluminescent method is being tested. To capture the air pollution particulate samples, various air filtering devices equipped with PTFE (Teflon) filters were allocated in different cities in Italy and left there for few days, then samples were collected and stored. Afterwards these filters were cut in halves and weighted, to find out the amount of particulate captured. To perform the assay, the filters were perforated with a paper puncher, creating small circles, which were distributed in the wells of a luminometer microplate and then the reagents’ mixture (100 μL) of hydrogen peroxide and luminol was added. The oxidizing free radicals present in the particulate react with the mixture triggering a light emission, recorded by the luminometer, which intensity should be proportional to the radicals’ amount. Evidences of a significant correlation between samples’ light signal and their toxicity are still under investigation. Preliminary results are very encouraging, suggesting that this luminescent assay can be a rapid method to estimate the toxicity of air particulates. [1] A. Filep, G.H. Fodor, F. Kun-Szabo, L. Tiszlavicz, Z. Razga, G. Bozso, Z, Bozoki, G. Szabo, F. Petak, Respir. Res. 17 (2016) 26. [2] M. Carugno, D. Consonni, G. Randi, D. Catelan, L. Grisotto, P.A. Bertazzi, A. Biggeri, M. Baccini, Environ. Res. 147 (2016) 415-424. [3] D. Schwela, Rev. Environ. Health 1-2 (2000) 13-42.
Development of a fast chemiluminescent method for testing air pollution
MORGANTI, EMANUELE;BOLELLI, LUCA;FERRI, ELIDA NORA;TOSITTI, LAURA;BRATTICH, ERIKA;GIROTTI, STEFANO
2016
Abstract
OB1. DEVELOPMENT OF A FAST CHEMILUMINESCENT METHOD FOR TESTING AIR POLLUTION Emanuele MORGANTI,1 Miguel AMORIM,2 Luca BOLELLI,1 Elida Nora FERRI,1 Laura TOSITTI,3 Erika BRATTICH,3 Stefano GIROTTI1 1Department of Pharmacy and Biotechnology FaBiT, Via San Donato 15, Alma Mater Studiorum - Università di Bologna, Bologna,40127, Italy 2Faculdade de Farmácia da Universidade de Lisboa, Avenida Prof. Gama Pinto Lisbon, 1649-003, Portugal 3Chemistry Department "Giacomo Ciamician" Via Selmi 2, Alma Mater Studiorum - Università di Bologna, Bologna, 40127, Italy Several epidemiological studies have evidenced the harmful effect of atmospheric particulate matter [1, 2]. The presence of certain substances, such as metals, WSOC (Water Soluble Organic Carbon), sulfur dioxide (SO2), nitrogen dioxide (NO2) and their highly oxidizing radicals SOx and NOx is strongly related to this toxicity [3]. In order to measure the concentration of oxidizing radicals in the air particulate, a chemiluminescent method is being tested. To capture the air pollution particulate samples, various air filtering devices equipped with PTFE (Teflon) filters were allocated in different cities in Italy and left there for few days, then samples were collected and stored. Afterwards these filters were cut in halves and weighted, to find out the amount of particulate captured. To perform the assay, the filters were perforated with a paper puncher, creating small circles, which were distributed in the wells of a luminometer microplate and then the reagents’ mixture (100 μL) of hydrogen peroxide and luminol was added. The oxidizing free radicals present in the particulate react with the mixture triggering a light emission, recorded by the luminometer, which intensity should be proportional to the radicals’ amount. Evidences of a significant correlation between samples’ light signal and their toxicity are still under investigation. Preliminary results are very encouraging, suggesting that this luminescent assay can be a rapid method to estimate the toxicity of air particulates. [1] A. Filep, G.H. Fodor, F. Kun-Szabo, L. Tiszlavicz, Z. Razga, G. Bozso, Z, Bozoki, G. Szabo, F. Petak, Respir. Res. 17 (2016) 26. [2] M. Carugno, D. Consonni, G. Randi, D. Catelan, L. Grisotto, P.A. Bertazzi, A. Biggeri, M. Baccini, Environ. Res. 147 (2016) 415-424. [3] D. Schwela, Rev. Environ. Health 1-2 (2000) 13-42.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
