In August 2015, in Bijeljina (Semberija province of Bosnia and Herzegovina) pepper and celery plants were surveyed for phytoplasma infections. During the survey pepper plants (Capsicum annuum ) of cvs Fortesa, Niška Šipka and Amanda showing stunting and leaf yellowing were observed, while celery plants (Apium graveolens L.) variety ‘Giant Prague’, expressed leaf whitening and stunting; the percentages of symptomatic plants ranged from 20 to 30%, respectively. Leaf samples from symptomatic and asymptomatic plants were test-ed to verify phytoplasma presence using nested-PCR/RFLP analyses. Results of 16S rDNA RFLP analyses with Tru1I and tuf typing using HpaII (Langer and Maixner, 2004) indicated the presence of a ‘Candidatus Phytoplasma solani’-related strain in all the symptomatic samples tested. There was no variability in the tuf gene and only tuf-type b was detected. Four symptomatic pepper and two celery samples were se-lected for further characterization amplifying vmp1 and stamp genes (Fialová et al., 2009; Fabre et al., 2011). The nucleotide sequences of the obtained amplicons were submitted to Gen-Bank (accession Nos. KU340846-51 and KU295501-06 for vmp1 and stamp sequences, respectively). Homology with ‘Ca. P. solani’ sequences from database was 96-100% for the vmp1 and 99-100% for the stamp gene. Combined RFLP analyses using Tru1I and Hpy188I on stamp and RsaI on vmp1 genes distinguished four ‘Ca. P. solani’ lineages indicating the pres-ence of genetic variability among the phytoplasmas studied. This is the first report of ‘Ca. P. solani’ presence in symptom-atic pepper and celery in Bosnia and Herzegovina.
First report of ‘Candidatus phytoplasma solani’ in pepper and celery in Bosnia and Herzegovina / Delić, D; Contaldo, N.; Lolić, B.; Moravčević, Đ.; Bertaccini, A.. - In: JOURNAL OF PLANT PATHOLOGY. - ISSN 1125-4653. - STAMPA. - 98:1(2016), pp. 184-184. [10.4454/JPP.V98I1.069]
First report of ‘Candidatus phytoplasma solani’ in pepper and celery in Bosnia and Herzegovina
CONTALDO, NICOLETTA;BERTACCINI, ASSUNTA
2016
Abstract
In August 2015, in Bijeljina (Semberija province of Bosnia and Herzegovina) pepper and celery plants were surveyed for phytoplasma infections. During the survey pepper plants (Capsicum annuum ) of cvs Fortesa, Niška Šipka and Amanda showing stunting and leaf yellowing were observed, while celery plants (Apium graveolens L.) variety ‘Giant Prague’, expressed leaf whitening and stunting; the percentages of symptomatic plants ranged from 20 to 30%, respectively. Leaf samples from symptomatic and asymptomatic plants were test-ed to verify phytoplasma presence using nested-PCR/RFLP analyses. Results of 16S rDNA RFLP analyses with Tru1I and tuf typing using HpaII (Langer and Maixner, 2004) indicated the presence of a ‘Candidatus Phytoplasma solani’-related strain in all the symptomatic samples tested. There was no variability in the tuf gene and only tuf-type b was detected. Four symptomatic pepper and two celery samples were se-lected for further characterization amplifying vmp1 and stamp genes (Fialová et al., 2009; Fabre et al., 2011). The nucleotide sequences of the obtained amplicons were submitted to Gen-Bank (accession Nos. KU340846-51 and KU295501-06 for vmp1 and stamp sequences, respectively). Homology with ‘Ca. P. solani’ sequences from database was 96-100% for the vmp1 and 99-100% for the stamp gene. Combined RFLP analyses using Tru1I and Hpy188I on stamp and RsaI on vmp1 genes distinguished four ‘Ca. P. solani’ lineages indicating the pres-ence of genetic variability among the phytoplasmas studied. This is the first report of ‘Ca. P. solani’ presence in symptom-atic pepper and celery in Bosnia and Herzegovina.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.