Quercetin is a well-investigated antioxidant known to protect cells against oxidative nuclear DNA damage. There is no knowledge regarding its effect on oxidative mitochondrial DNA damage. In this study we investigated the effect of quercetin on oxidatively-injured DNA. Cell-free and cell studies were performed. Cell-free analyses carried out on plasmidic DNA showed that quercetin protects from all oxidative challenges used. Cellular studies were carried out on NCTC 2544 cells which were insulted with hydrogen peroxide and UVC radiations. Nuclear and mitochondrial DNAs were analysed by measuring DNA damage with a quantitative polymerase chain reaction. Quercetin supplementation showed significant genoprotective activity on mitochondrial DNA when hydroperoxide was used. The evidence of the protection afforded by quercetin suggests that this flavonoid may play an important role on mitochondrial genome stability. © 2008 IUBMB/IOS Press and the authors. All rights reserved.

Effect of quercetin on oxidative nuclear and mitochondrial DNA damage / Potenza, Lucia; Calcabrini, Cinzia; De Bellis, Roberta; Mancini, Umberto; Cucchiarini, Luigi; Dach, Marina. - In: BIOFACTORS. - ISSN 0951-6433. - ELETTRONICO. - 33:1(2008), pp. 33-48. [10.1002/biof.5520330104]

Effect of quercetin on oxidative nuclear and mitochondrial DNA damage

CALCABRINI, CINZIA;
2008

Abstract

Quercetin is a well-investigated antioxidant known to protect cells against oxidative nuclear DNA damage. There is no knowledge regarding its effect on oxidative mitochondrial DNA damage. In this study we investigated the effect of quercetin on oxidatively-injured DNA. Cell-free and cell studies were performed. Cell-free analyses carried out on plasmidic DNA showed that quercetin protects from all oxidative challenges used. Cellular studies were carried out on NCTC 2544 cells which were insulted with hydrogen peroxide and UVC radiations. Nuclear and mitochondrial DNAs were analysed by measuring DNA damage with a quantitative polymerase chain reaction. Quercetin supplementation showed significant genoprotective activity on mitochondrial DNA when hydroperoxide was used. The evidence of the protection afforded by quercetin suggests that this flavonoid may play an important role on mitochondrial genome stability. © 2008 IUBMB/IOS Press and the authors. All rights reserved.
2008
Effect of quercetin on oxidative nuclear and mitochondrial DNA damage / Potenza, Lucia; Calcabrini, Cinzia; De Bellis, Roberta; Mancini, Umberto; Cucchiarini, Luigi; Dach, Marina. - In: BIOFACTORS. - ISSN 0951-6433. - ELETTRONICO. - 33:1(2008), pp. 33-48. [10.1002/biof.5520330104]
Potenza, Lucia; Calcabrini, Cinzia; De Bellis, Roberta; Mancini, Umberto; Cucchiarini, Luigi; Dach, Marina
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/594437
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