A invertebrate DNA microarray targeting ten species common in the Mediterranean was developed within the framework of the FP6 STREP FISH& CHIPS. Based on sequence variation of the 16S rDNA gene, we designed species-specific probes that were used to construct a DNA chip prototype for the identification of invertebrate (i.e. molluscans and crustaceans) preys of demersal fishes in the Adriatic Sea. The constructed invertebrate DNA chip represents a technological advance towards the wide use of rapid and high-resolution molecular tools to assess structure and changes of marine ecosystems such as the monitoring of invasive species and of re-emerging pathogens in ballast waters.
A. Cariani, M. Landi, F. Bertasi, M. Colangelo, V.U. Ceccherelli, F. Tinti, et al. (2007). DEVELOPMENT OF HIGH-RESOLUTION MOLECULAR TECHNOLOGIES TO INVESTIGATE ECOSYSTEM CHANGES IN THE SOUTHERN EUROPEAN SEAS: THE PROTOTYPING OF DNA CHIPS TO IDENTIFY INVERTEBRATE PREY SPECIES IN MARINE FOOD WEBS OF ADRIATIC SEA.
DEVELOPMENT OF HIGH-RESOLUTION MOLECULAR TECHNOLOGIES TO INVESTIGATE ECOSYSTEM CHANGES IN THE SOUTHERN EUROPEAN SEAS: THE PROTOTYPING OF DNA CHIPS TO IDENTIFY INVERTEBRATE PREY SPECIES IN MARINE FOOD WEBS OF ADRIATIC SEA
CARIANI, ALESSIA;LANDI, MONICA;COLANGELO, MARINA ANTONIA;TINTI, FAUSTO;
2007
Abstract
A invertebrate DNA microarray targeting ten species common in the Mediterranean was developed within the framework of the FP6 STREP FISH& CHIPS. Based on sequence variation of the 16S rDNA gene, we designed species-specific probes that were used to construct a DNA chip prototype for the identification of invertebrate (i.e. molluscans and crustaceans) preys of demersal fishes in the Adriatic Sea. The constructed invertebrate DNA chip represents a technological advance towards the wide use of rapid and high-resolution molecular tools to assess structure and changes of marine ecosystems such as the monitoring of invasive species and of re-emerging pathogens in ballast waters.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
