A invertebrate DNA microarray targeting ten species common in the Mediterranean was developed within the framework of the FP6 STREP FISH& CHIPS. Based on sequence variation of the 16S rDNA gene, we designed species-specific probes that were used to construct a DNA chip prototype for the identification of invertebrate (i.e. molluscans and crustaceans) preys of demersal fishes in the Adriatic Sea. The constructed invertebrate DNA chip represents a technological advance towards the wide use of rapid and high-resolution molecular tools to assess structure and changes of marine ecosystems such as the monitoring of invasive species and of re-emerging pathogens in ballast waters.

DEVELOPMENT OF HIGH-RESOLUTION MOLECULAR TECHNOLOGIES TO INVESTIGATE ECOSYSTEM CHANGES IN THE SOUTHERN EUROPEAN SEAS: THE PROTOTYPING OF DNA CHIPS TO IDENTIFY INVERTEBRATE PREY SPECIES IN MARINE FOOD WEBS OF ADRIATIC SEA

CARIANI, ALESSIA;LANDI, MONICA;COLANGELO, MARINA ANTONIA;TINTI, FAUSTO;
2007

Abstract

A invertebrate DNA microarray targeting ten species common in the Mediterranean was developed within the framework of the FP6 STREP FISH& CHIPS. Based on sequence variation of the 16S rDNA gene, we designed species-specific probes that were used to construct a DNA chip prototype for the identification of invertebrate (i.e. molluscans and crustaceans) preys of demersal fishes in the Adriatic Sea. The constructed invertebrate DNA chip represents a technological advance towards the wide use of rapid and high-resolution molecular tools to assess structure and changes of marine ecosystems such as the monitoring of invasive species and of re-emerging pathogens in ballast waters.
2007
444
444
A. Cariani; M. Landi; F. Bertasi; M. Colangelo; V.U. Ceccherelli; F. Tinti; S. Chitipothu; D. Blohm
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/59327
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