Most mammalian cells express a wide range of potassium (K+) channels, such as large conductance K+ (BK) and voltage gated K+ (Kv) channels. In non-excitable cells, K+ channels are involved in volume regulation, hormonal secretion, cell proliferation, migration and apoptosis [1,2]. The ability of ion channels to act as modulators of conductance is due to their innate property of rapid conformational alterations to initiate or respond to signal transduction [3]. Several lines of evidence from molecular, biochemical, and biological studies outline that different pathways are operating in cells and organisms at low and high radiation doses leading to a non linearity in the dose–response increase. A previous study performed in human cell lines showed that both normal and cancer cells exhibit an increase in Kv currents after treatment with low dose ionizing radiation (LDIR), suggesting that K+ current (IK) modifications are acting as a component of a signal transduction pathway(s) in response to stress, such as amplification of reactive oxygen species (ROS) signaling cascade [4]. Moreover, it has been shown that ionizing radiation (IR) might directly modify BK channel activity and therefore modulate cytosolic free Ca++ concentration and CAMKII pathway [5]. In this work, we studied the effects of proton (H+) irradiation at different doses (from 0.25 to 4 Gy) on wholecell IK and gene expression in a rodent and a human cell system: Chinese hamster lung fibroblasts (V79) and glioblastoma multiform cell line (T98G) respectively.
Zironi, I., Gavoçi, E., Virelli, A., Lanzarini, C., Capri, M., Remondini, D., et al. (2011). Effects of Proton Irradiation on Ionic Currents and Gene Expression in Human and Rodent Cell Lines. Legnaro (PD) : Paola Spolaore; Sara Carturan; Mario Maggiore; Giuseppe Ruoso; Anna D’Este.
Effects of Proton Irradiation on Ionic Currents and Gene Expression in Human and Rodent Cell Lines
ZIRONI, ISABELLA;GAVOCI, ENTELE;VIRELLI, ANGELA;LANZARINI, CATIA;CAPRI, MIRIAM;REMONDINI, DANIEL;CASTELLANI, GASTONE
2011
Abstract
Most mammalian cells express a wide range of potassium (K+) channels, such as large conductance K+ (BK) and voltage gated K+ (Kv) channels. In non-excitable cells, K+ channels are involved in volume regulation, hormonal secretion, cell proliferation, migration and apoptosis [1,2]. The ability of ion channels to act as modulators of conductance is due to their innate property of rapid conformational alterations to initiate or respond to signal transduction [3]. Several lines of evidence from molecular, biochemical, and biological studies outline that different pathways are operating in cells and organisms at low and high radiation doses leading to a non linearity in the dose–response increase. A previous study performed in human cell lines showed that both normal and cancer cells exhibit an increase in Kv currents after treatment with low dose ionizing radiation (LDIR), suggesting that K+ current (IK) modifications are acting as a component of a signal transduction pathway(s) in response to stress, such as amplification of reactive oxygen species (ROS) signaling cascade [4]. Moreover, it has been shown that ionizing radiation (IR) might directly modify BK channel activity and therefore modulate cytosolic free Ca++ concentration and CAMKII pathway [5]. In this work, we studied the effects of proton (H+) irradiation at different doses (from 0.25 to 4 Gy) on wholecell IK and gene expression in a rodent and a human cell system: Chinese hamster lung fibroblasts (V79) and glioblastoma multiform cell line (T98G) respectively.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
