Oligo(Glu70-co-Leu30), a peptide synthesized by protease catalysis, is functionalized at the N-terminus with a 4-pentenoyl unit and grafted to polyLSL[6′Ac,6″Ac], a glycopolymer prepared by ring-opening metathesis polymerization of lactonic sophorolipid diacetate. First, polyLSL[6'Ac,6”Ac] fiber mats are fabricated by electrospinning. Oxidation of the fiber mats and subsequent reaction with cysteamine lead to thiol-functionalized fiber mats with no significant morphology changes. Grafting of the alkene-modified oligopeptide to thiol-functionalized polyLSL[6′Ac,6″Ac] fiber mats is achieved via “thiol-ene” click reaction. X-ray photoelectron spectroscopy analysis to characterize peptide grafting reveals that about 50 mol% of polyLSL[6′Ac,6′′Ac] repeat units at fiber surfaces are decorated with a peptide moiety, out of which about 1/3 of the oligo(Glu70-co-Leu30) units are physically adsorbed to polyLSL[6′Ac,6′′Ac]. The results of this work pave the way to precise engineering of polyLSL fiber mats that can be decorated with a potentially wide range of molecules that tailor surface chemistry and biological properties. (Figure presented.).

Fiorani, A., Totsingan, F., Pollicino, A., Peng, Y., Focarete, M.L., Gross, R.A., et al. (2017). Peptide Modified Electrospun Glycopolymer Fibers. MACROMOLECULAR BIOSCIENCE, 17(3), 1-12 [10.1002/mabi.201600327].

Peptide Modified Electrospun Glycopolymer Fibers

FIORANI, ANDREA;FOCARETE, MARIA LETIZIA;SCANDOLA, MARIASTELLA
2017

Abstract

Oligo(Glu70-co-Leu30), a peptide synthesized by protease catalysis, is functionalized at the N-terminus with a 4-pentenoyl unit and grafted to polyLSL[6′Ac,6″Ac], a glycopolymer prepared by ring-opening metathesis polymerization of lactonic sophorolipid diacetate. First, polyLSL[6'Ac,6”Ac] fiber mats are fabricated by electrospinning. Oxidation of the fiber mats and subsequent reaction with cysteamine lead to thiol-functionalized fiber mats with no significant morphology changes. Grafting of the alkene-modified oligopeptide to thiol-functionalized polyLSL[6′Ac,6″Ac] fiber mats is achieved via “thiol-ene” click reaction. X-ray photoelectron spectroscopy analysis to characterize peptide grafting reveals that about 50 mol% of polyLSL[6′Ac,6′′Ac] repeat units at fiber surfaces are decorated with a peptide moiety, out of which about 1/3 of the oligo(Glu70-co-Leu30) units are physically adsorbed to polyLSL[6′Ac,6′′Ac]. The results of this work pave the way to precise engineering of polyLSL fiber mats that can be decorated with a potentially wide range of molecules that tailor surface chemistry and biological properties. (Figure presented.).
2017
Fiorani, A., Totsingan, F., Pollicino, A., Peng, Y., Focarete, M.L., Gross, R.A., et al. (2017). Peptide Modified Electrospun Glycopolymer Fibers. MACROMOLECULAR BIOSCIENCE, 17(3), 1-12 [10.1002/mabi.201600327].
Fiorani, Andrea; Totsingan, Filbert; Pollicino, Antonio; Peng, Yifeng; Focarete, Maria Letizia; Gross, Richard A; Scandola, Mariastella
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/586392
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