Live cell imaging can provide important information on cellular dynamics; however, the full utilisation of this technology has been hampered by the limitations of imaging reagents. Metal-based complexes have the potential to overcome many of the issues common to many current imaging agents. The rhenium (I)-based complex fac-[Re(CO)3(1,10-phenanthroline)(4-pyridyltetrazolate)], herein referred to as ReZolve-ER™, shows promise as a live cell imaging agent with rapid cell uptake, low cytotoxicity, resistance to photobleaching and compatibility with multicolour imaging. ReZolve-ER™ localised to the nuclear membrane/endoplasmic reticulum (ER) and allowed the detection of exocytotic events at the plasma membrane. Thus, we present a new imaging agent for monitoring live cell events in real time, which is ideal for imaging either short- or long-time courses.
Imaging nuclear, endoplasmic reticulum and plasma membrane events in real time / Bader, Christie A.; Sorvina, Alexandra; Simpson, Peter V.; Wright, Phillip J.; Stagni, Stefano; Plush, Sally E.; Massi, Massimiliano; Brooks, Douglas A. - In: FEBS LETTERS. - ISSN 0014-5793. - STAMPA. - 590:18(2016), pp. 3051-3060. [10.1002/1873-3468.12365]
Imaging nuclear, endoplasmic reticulum and plasma membrane events in real time
STAGNI, STEFANO;MASSI, MASSIMILIANO;
2016
Abstract
Live cell imaging can provide important information on cellular dynamics; however, the full utilisation of this technology has been hampered by the limitations of imaging reagents. Metal-based complexes have the potential to overcome many of the issues common to many current imaging agents. The rhenium (I)-based complex fac-[Re(CO)3(1,10-phenanthroline)(4-pyridyltetrazolate)], herein referred to as ReZolve-ER™, shows promise as a live cell imaging agent with rapid cell uptake, low cytotoxicity, resistance to photobleaching and compatibility with multicolour imaging. ReZolve-ER™ localised to the nuclear membrane/endoplasmic reticulum (ER) and allowed the detection of exocytotic events at the plasma membrane. Thus, we present a new imaging agent for monitoring live cell events in real time, which is ideal for imaging either short- or long-time courses.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
