Abstract Staphylococcus aureus is the leading etiologic agent of orthopedic implant infections. Here a ribocluster of 27 S. aureus strains underwent further molecular characterization and subtyping by multilocus sequence typing (MLST) and spa-typing. This cluster had been detected by automated ribotyping (with the EcoRl restriction enzyme) of 200 S. aureus isolates from periprosthetic infections of patients who underwent revision at the Rizzoli Orthopaedic Institute. The ribocluster, consisting of agr type III strains, with a 74% co-occurrence of bone sialoprotein-binding (bbp) and collagen-binding (cna) genes, lacked mecA and IS256, and exhibited a high prevalence of the toxic shock syndrome toxin gene (tst, 85%). Strains' relatedness was analyzed by BURP and eBURST. Two predominant spa types, t012 (32%) and t021 (36%), and one predominant sequence type, ST30 (18/27, 67%) were identified: a S. aureus lineage spread worldwide belonging to MLST CC30. Two new sequence types (ST2954, ST2960) and one new spa type (t13129) were detected for the first time. Interestingly, the 27-strain cluster detected by ribotyping corresponded exactly to MLST CC30, the sole CC identified by eBURST.

Montanaro, L., Ravaioli, S., Ruppitsch, W., Campoccia, D., Pietrocola, G., Visai, L., et al. (2016). Molecular Characterization of a Prevalent Ribocluster of Methicillin-Sensitive Staphylococcus aureus from Orthopedic Implant Infections. Correspondence with MLST CC30. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, 6, 1-11 [10.3389/fcimb.2016.00008].

Molecular Characterization of a Prevalent Ribocluster of Methicillin-Sensitive Staphylococcus aureus from Orthopedic Implant Infections. Correspondence with MLST CC30

MONTANARO, LUCIO;RAVAIOLI, STEFANO;ARCIOLA, CARLA RENATA
2016

Abstract

Abstract Staphylococcus aureus is the leading etiologic agent of orthopedic implant infections. Here a ribocluster of 27 S. aureus strains underwent further molecular characterization and subtyping by multilocus sequence typing (MLST) and spa-typing. This cluster had been detected by automated ribotyping (with the EcoRl restriction enzyme) of 200 S. aureus isolates from periprosthetic infections of patients who underwent revision at the Rizzoli Orthopaedic Institute. The ribocluster, consisting of agr type III strains, with a 74% co-occurrence of bone sialoprotein-binding (bbp) and collagen-binding (cna) genes, lacked mecA and IS256, and exhibited a high prevalence of the toxic shock syndrome toxin gene (tst, 85%). Strains' relatedness was analyzed by BURP and eBURST. Two predominant spa types, t012 (32%) and t021 (36%), and one predominant sequence type, ST30 (18/27, 67%) were identified: a S. aureus lineage spread worldwide belonging to MLST CC30. Two new sequence types (ST2954, ST2960) and one new spa type (t13129) were detected for the first time. Interestingly, the 27-strain cluster detected by ribotyping corresponded exactly to MLST CC30, the sole CC identified by eBURST.
2016
Montanaro, L., Ravaioli, S., Ruppitsch, W., Campoccia, D., Pietrocola, G., Visai, L., et al. (2016). Molecular Characterization of a Prevalent Ribocluster of Methicillin-Sensitive Staphylococcus aureus from Orthopedic Implant Infections. Correspondence with MLST CC30. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, 6, 1-11 [10.3389/fcimb.2016.00008].
Montanaro, L; Ravaioli, S; Ruppitsch, W; Campoccia, D; Pietrocola, G; Visai, L; Speziale, P; Allerberger, F; Arciola, Cr
File in questo prodotto:
File Dimensione Formato  
fcimb-06-00008.pdf

accesso aperto

Tipo: Versione (PDF) editoriale
Licenza: Licenza per Accesso Aperto. Creative Commons Attribuzione (CCBY)
Dimensione 1.2 MB
Formato Adobe PDF
1.2 MB Adobe PDF Visualizza/Apri
Table1.doc

accesso aperto

Descrizione: GUID: 3CF8B2DD-A288-4DB9-809D-076A6416F26A
Tipo: File Supplementare
Licenza: Licenza per Accesso Aperto. Altra tipologia di licenza compatibile con Open Access
Dimensione 100 kB
Formato Microsoft Word
100 kB Microsoft Word Visualizza/Apri
Table2.doc

accesso aperto

Descrizione: GUID: 50FFEB0C-D493-4F95-815F-F7D54D775911
Tipo: File Supplementare
Licenza: Licenza per Accesso Aperto. Altra tipologia di licenza compatibile con Open Access
Dimensione 36.5 kB
Formato Microsoft Word
36.5 kB Microsoft Word Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/566295
Citazioni
  • ???jsp.display-item.citation.pmc??? 11
  • Scopus 17
  • ???jsp.display-item.citation.isi??? 20
social impact