Recovery of antibodies with Protein A affinity chromatography columns has become the standard for the biotech industry. Membrane affinity chromatography has never taken off the ground due to the lower capacity of membrane supports compared to chromatographic beads. In this work new affinity membranes endowed with high capacity for IgG will be presented. These membranes have been experimentally characterized using a novel approach that integrates experiments with a mathematical modelling. All the relevant parameters like static and dynamic binding capacity, selectivity and purity of the recovered antibody have been evaluated and will be discussed. Indications towards scale-up and comparison with the state of the art of affinity chromatography will be addressed.

Advances in membrane affinity chromatography for the recovery of antibodies

BOI, CRISTIANA;DIMARTINO, SIMONE;SARTI, GIULIO CESARE
2007

Abstract

Recovery of antibodies with Protein A affinity chromatography columns has become the standard for the biotech industry. Membrane affinity chromatography has never taken off the ground due to the lower capacity of membrane supports compared to chromatographic beads. In this work new affinity membranes endowed with high capacity for IgG will be presented. These membranes have been experimentally characterized using a novel approach that integrates experiments with a mathematical modelling. All the relevant parameters like static and dynamic binding capacity, selectivity and purity of the recovered antibody have been evaluated and will be discussed. Indications towards scale-up and comparison with the state of the art of affinity chromatography will be addressed.
BIOT - ACS
C. Boi; S. Dimartino; G. C. Sarti
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11585/56321
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