BACKGROUND: Little is known regarding the potentially chemopreventive activity of cyanidin-3-O-beta-glucopyranoside (Cy-g), the main anthocyanin present in the juice of pigmented oranges, apart from its antioxidant activity. After excluding a potential genotoxicity of Cy-g, its ability to induce apoptosis on transformed and normal T cells was analysed. In order to delineate the events leading to apoptosis, the expression of different proteins, known to be involved in apoptosis, was also measured. MATERIALS AND METHODS: The evaluation of genotoxicity was performed by the micronucleus test. Flow cytometry was used for the analysis of apoptotic cells and proteins involved in the modulation of apoptosis. RESULTS: Cy-g was nongenotoxic. Moreover, it induced apoptosis in both cell systems, modulated by an increase of p53 and bax proteins. CONCLUSION: These interesting biological properties should encourage further studies into the chemopreventive potential of Cy-g. Nevertheless, its activity in normal T cells underlines the need for extensive toxicological investigation.

Fimognari C., Berti F., Nuesse M., Cantelli Forti G., Hrelia P. (2005). In vitro anticancer activity of cyanidin-3-O-beta-glucopyranoside: effects on transformed and non-transformed T lymphocytes. ANTICANCER RESEARCH, 25, 2837-2840.

In vitro anticancer activity of cyanidin-3-O-beta-glucopyranoside: effects on transformed and non-transformed T lymphocytes.

FIMOGNARI, CARMELA;CANTELLI FORTI, GIORGIO;HRELIA, PATRIZIA
2005

Abstract

BACKGROUND: Little is known regarding the potentially chemopreventive activity of cyanidin-3-O-beta-glucopyranoside (Cy-g), the main anthocyanin present in the juice of pigmented oranges, apart from its antioxidant activity. After excluding a potential genotoxicity of Cy-g, its ability to induce apoptosis on transformed and normal T cells was analysed. In order to delineate the events leading to apoptosis, the expression of different proteins, known to be involved in apoptosis, was also measured. MATERIALS AND METHODS: The evaluation of genotoxicity was performed by the micronucleus test. Flow cytometry was used for the analysis of apoptotic cells and proteins involved in the modulation of apoptosis. RESULTS: Cy-g was nongenotoxic. Moreover, it induced apoptosis in both cell systems, modulated by an increase of p53 and bax proteins. CONCLUSION: These interesting biological properties should encourage further studies into the chemopreventive potential of Cy-g. Nevertheless, its activity in normal T cells underlines the need for extensive toxicological investigation.
2005
Fimognari C., Berti F., Nuesse M., Cantelli Forti G., Hrelia P. (2005). In vitro anticancer activity of cyanidin-3-O-beta-glucopyranoside: effects on transformed and non-transformed T lymphocytes. ANTICANCER RESEARCH, 25, 2837-2840.
Fimognari C.; Berti F.; Nuesse M.; Cantelli Forti G.; Hrelia P.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/5619
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