The faecal microbiota composition of infants born to mothers receiving intrapartum antibiotic prophylaxis with ampicillin against group B Streptococcus was compared with that of control infants, at day 7 and 30 of life. Recruited newborns were both exclusive breastfed and mixed fed, in order to also study the effect of dietary factors on the microbiota composition. Massive parallel sequencing of the V3-V4 region of the 16S rRNA gene and qPCR analysis were performed. Antibiotic prophylaxis caused the most marked changes on the microbiota in breastfed infants, mainly resulting in a higher relative abundance of Enterobacteriaceae, compared with control infants (52% vs. 14%, p = 0.044) and mixed-fed infants (52% vs. 16%, p = 0.13 NS) at day 7 and in a lower bacterial diversity compared to mixed-fed infants and controls. Bifidobacteria were also particularly vulnerable and abundances were reduced in breastfed (p = 0.001) and mixed-fed antibiotic treated groups compared to nontreated groups. Reductions in bifidobacteria in antibiotic treated infants were also confirmed by qPCR. By day 30, the bifidobacterial population recovered and abundances significantly increased in both breastfed (p = 0.025) and mixed-fed (p = 0.013) antibiotic treated groups, whereas Enterobacteriaceae abundances remained highest in the breastfed antibiotic treated group (44%), compared with control infants (16%) and mixed-fed antibiotic treated group (28%). This study has therefore demonstrated the short term consequences of maternal intrapartum antibiotic prophylaxis on the infant faecal microbial population, particularly in that of breastfed infants.

Early gut microbiota perturbations following intrapartum antibiotic prophylaxis to prevent group B streptococcal disease / Giuseppe Mazzola; Kiera Murphy; R. Paul Ross; Diana Di Gioia; Bruno Biavati; Luigi T. Corvaglia; Giacomo Faldella; Catherine Stanton. - In: PLOS ONE. - ISSN 1932-6203. - STAMPA. - 11:6(2016), pp. e0157527.1-e0157527.11. [10.1371/journal.pone.0157527]

Early gut microbiota perturbations following intrapartum antibiotic prophylaxis to prevent group B streptococcal disease

MAZZOLA, GIUSEPPE;DI GIOIA, DIANA;BIAVATI, BRUNO;CORVAGLIA, LUIGI TOMMASO;FALDELLA, GIACOMO;
2016

Abstract

The faecal microbiota composition of infants born to mothers receiving intrapartum antibiotic prophylaxis with ampicillin against group B Streptococcus was compared with that of control infants, at day 7 and 30 of life. Recruited newborns were both exclusive breastfed and mixed fed, in order to also study the effect of dietary factors on the microbiota composition. Massive parallel sequencing of the V3-V4 region of the 16S rRNA gene and qPCR analysis were performed. Antibiotic prophylaxis caused the most marked changes on the microbiota in breastfed infants, mainly resulting in a higher relative abundance of Enterobacteriaceae, compared with control infants (52% vs. 14%, p = 0.044) and mixed-fed infants (52% vs. 16%, p = 0.13 NS) at day 7 and in a lower bacterial diversity compared to mixed-fed infants and controls. Bifidobacteria were also particularly vulnerable and abundances were reduced in breastfed (p = 0.001) and mixed-fed antibiotic treated groups compared to nontreated groups. Reductions in bifidobacteria in antibiotic treated infants were also confirmed by qPCR. By day 30, the bifidobacterial population recovered and abundances significantly increased in both breastfed (p = 0.025) and mixed-fed (p = 0.013) antibiotic treated groups, whereas Enterobacteriaceae abundances remained highest in the breastfed antibiotic treated group (44%), compared with control infants (16%) and mixed-fed antibiotic treated group (28%). This study has therefore demonstrated the short term consequences of maternal intrapartum antibiotic prophylaxis on the infant faecal microbial population, particularly in that of breastfed infants.
2016
Early gut microbiota perturbations following intrapartum antibiotic prophylaxis to prevent group B streptococcal disease / Giuseppe Mazzola; Kiera Murphy; R. Paul Ross; Diana Di Gioia; Bruno Biavati; Luigi T. Corvaglia; Giacomo Faldella; Catherine Stanton. - In: PLOS ONE. - ISSN 1932-6203. - STAMPA. - 11:6(2016), pp. e0157527.1-e0157527.11. [10.1371/journal.pone.0157527]
Giuseppe Mazzola; Kiera Murphy; R. Paul Ross; Diana Di Gioia; Bruno Biavati; Luigi T. Corvaglia; Giacomo Faldella; Catherine Stanton
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Descrizione: S1 Fig: Evaluation of alpha diversity. Estimates of alpha diversity for faecal samples from BF-IAP, BF-C, MF-IAP cohorts at day 30.
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Descrizione: S2 Fig: Abundance of bacterial families. Relative abundances of bacterial families in BF-IAP, BF-C, MF-IAP and MF-C faecal samples. The Other category contains all other families present at <1% of relative abundance. Black and white colour in print is required
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/561102
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