Hemp (Cannabis sativa) is a sustainable crop well adapted to most European conditions. It is traditionally cultivated for fibres, seeds, psychoactive substances and recently evaluated as ideal crop to produce innovative biomaterials. It has a reputation for being resistant to stresses and diseases, although it hosts some insects besides fungal and viral pathogens. Among viral diseases, Alfalfa mosaic virus, Cucumber mosaic virus and Arabis mosaic virus have been detected in hemp. Other viral symptoms, described as yellow streak, wrinkled leaf margins and leaf tips rolled upwards, have been observed in Europe since 1941 and attributed to a causal agent named Hemp Streak Virus. At CRA-CIN those symptoms have been observed on several varieties since many years and experimental evidence suggests that they are not transmitted thorough grafting or mechanical inoculation, do not have apparent fungal or insect vector and are transmitted only vertically through pollen and seed. Meanwhile, the Cannabis cryptic virus (CCV), family Partitiviridae, was isolated from a French hemp accession and its complete sequence recently published. Plant cryptoviruses are transmitted vertically and are usually persistent and asymptomatic, except for few cases where their presence was tentatively related to symptoms. Aim of this work was to verify the role of CCV in the observed symptoms in hemp. A total of 35 hemp varieties available at CRA-CIN within the frame of the FP7-Multihemp project were phenotyped for viral symptoms and analysed by TaqMan assay to determine presence and titre of CCV. Results are discussed in relation to Koch’s postulates.
Paris, R., Righetti, L., Calzolari, D., Onofri, C., Pacifico, D., Magagnini, G., et al. (2014). HOW CRYPTIC IS THE CANNABIS CRYPTIC VIRUS? [10.4454/jpp.v96i2SUP.3301].
HOW CRYPTIC IS THE CANNABIS CRYPTIC VIRUS?
RATTI, CLAUDIO;
2014
Abstract
Hemp (Cannabis sativa) is a sustainable crop well adapted to most European conditions. It is traditionally cultivated for fibres, seeds, psychoactive substances and recently evaluated as ideal crop to produce innovative biomaterials. It has a reputation for being resistant to stresses and diseases, although it hosts some insects besides fungal and viral pathogens. Among viral diseases, Alfalfa mosaic virus, Cucumber mosaic virus and Arabis mosaic virus have been detected in hemp. Other viral symptoms, described as yellow streak, wrinkled leaf margins and leaf tips rolled upwards, have been observed in Europe since 1941 and attributed to a causal agent named Hemp Streak Virus. At CRA-CIN those symptoms have been observed on several varieties since many years and experimental evidence suggests that they are not transmitted thorough grafting or mechanical inoculation, do not have apparent fungal or insect vector and are transmitted only vertically through pollen and seed. Meanwhile, the Cannabis cryptic virus (CCV), family Partitiviridae, was isolated from a French hemp accession and its complete sequence recently published. Plant cryptoviruses are transmitted vertically and are usually persistent and asymptomatic, except for few cases where their presence was tentatively related to symptoms. Aim of this work was to verify the role of CCV in the observed symptoms in hemp. A total of 35 hemp varieties available at CRA-CIN within the frame of the FP7-Multihemp project were phenotyped for viral symptoms and analysed by TaqMan assay to determine presence and titre of CCV. Results are discussed in relation to Koch’s postulates.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
