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EnglishJ Biomed Mater Res A. A multiplex PCR method for the detection of all five individual genes of ica locus in Staphylococcus epidermidis. A survey on 400 clinical isolates from prosthesis-associated infections. Arciola CR, Gamberini S, Campoccia D, Visai L, Speziale P, Baldassarri L, Montanaro L. Research Unit on Implant Infections, Rizzoli Orthopedic Institute, Via di Barbiano, 1/10, 40136 Bologna, Italy. carlarenata.arciola@ior.it In Staphylococcus epidermidis, ica locus encodes for the synthesis of a polysaccharide intercellular adhesin (slime or biofilm). A multiplex polymerase chain reaction (PCR) for the detection of the five individual genes of ica locus was developed, with the aim to probe the set of genes in a large collection of Staphylococcus epidermidis clinical isolates. Single representative fragments for icaR, icaA, icaD, icaB, and icaC genes were selected. Multiplex PCR was applied to two reference Staphylococcus epidermidis strains [the non-biofilm-forming ATCC 12228 and the biofilm-forming ATCC 35984 (RP62A)] and to 400 clinical isolates of Staphylococcus epidermidis from orthopedic prosthesis associated infections. The gene profile was compared with the phenotypic biofilm-forming ability, evaluated by means of an optimized Congo red agar (CRA) plate test. Among the clinical isolates, 228 (57%) turned out completely ica positive and were biofilm producing. Among the 172 non-biofilm-forming strains (43%), 164 (41%) were completely ica negative and 8 strains (2%) harbored all five ica genes. The ica locus thus proves to be a cluster of strictly linked genes, without any evidence of single gene deletion. (c) 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2005.
| Titolo: | A multiplex PCR method for the detection of all five individual genes of ica locus in Staphylococcus epidermidis. A survey on 400 clinical isolates from prosthesis-associated infections | |
| Autore/i: | ARCIOLA, CARLA RENATA; Gamberini S; Campoccia D; Visai L; Speziale P; Baldassarri L; MONTANARO, LUCIO | |
| Autore/i Unibo: | ||
| Anno: | 2005 | |
| Rivista: | ||
| Digital Object Identifier (DOI): | http://dx.doi.org/10.1002/jbm.a.30445 | |
| Abstract: | J Biomed Mater Res A. A multiplex PCR method for the detection of all five individual genes of ica locus in Staphylococcus epidermidis. A survey on 400 clinical isolates from prosthesis-associated infections. Arciola CR, Gamberini S, Campoccia D, Visai L, Speziale P, Baldassarri L, Montanaro L. Research Unit on Implant Infections, Rizzoli Orthopedic Institute, Via di Barbiano, 1/10, 40136 Bologna, Italy. carlarenata.arciola@ior.it In Staphylococcus epidermidis, ica locus encodes for the synthesis of a polysaccharide intercellular adhesin (slime or biofilm). A multiplex polymerase chain reaction (PCR) for the detection of the five individual genes of ica locus was developed, with the aim to probe the set of genes in a large collection of Staphylococcus epidermidis clinical isolates. Single representative fragments for icaR, icaA, icaD, icaB, and icaC genes were selected. Multiplex PCR was applied to two reference Staphylococcus epidermidis strains [the non-biofilm-forming ATCC 12228 and the biofilm-forming ATCC 35984 (RP62A)] and to 400 clinical isolates of Staphylococcus epidermidis from orthopedic prosthesis associated infections. The gene profile was compared with the phenotypic biofilm-forming ability, evaluated by means of an optimized Congo red agar (CRA) plate test. Among the clinical isolates, 228 (57%) turned out completely ica positive and were biofilm producing. Among the 172 non-biofilm-forming strains (43%), 164 (41%) were completely ica negative and 8 strains (2%) harbored all five ica genes. The ica locus thus proves to be a cluster of strictly linked genes, without any evidence of single gene deletion. (c) 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2005. | |
| Data prodotto definitivo in UGOV: | 2005-10-16 17:42:25 | |
| Appare nelle tipologie: | 1.01 Articolo in rivista |
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