The objective of this studywas to develop a rapidmethod to isolateDNA fromVenturia inaequalis spores for use in diagnostic DNA mutation analysis. Chelex-100 resin was evaluated and compared with a well established DNA exctraction method, utilizing CTAB in order to have a robust comparison. In this research we demonstrated that Chelex-100 efficiently makes extraction of the DNA from V. inaequalis spores available for direct use inmolecular analyses. Also, the quantity and quality of extracted DNA were shown to be adequate for PCR analysis. Comparatively, the quality of DNA samples isolated using Chelex method was better than those extracted using CTAB. In conclusion, the Chelexmethod is recommended for PCR experiments considering its simplicity and costeffectiveness.
Turan, C., Nanni, I.M., Brunelli, A., Collina, M. (2015). New Rapid DNA extraction method with Chelex from Venturia inaequalis spores. JOURNAL OF MICROBIOLOGICAL METHODS, 115, 139-143 [10.1016/j.mimet.2015.06.005].
New Rapid DNA extraction method with Chelex from Venturia inaequalis spores
TURAN, CEREN;NANNI, IRENE MAJA;BRUNELLI, AGOSTINO;COLLINA, MARINA
2015
Abstract
The objective of this studywas to develop a rapidmethod to isolateDNA fromVenturia inaequalis spores for use in diagnostic DNA mutation analysis. Chelex-100 resin was evaluated and compared with a well established DNA exctraction method, utilizing CTAB in order to have a robust comparison. In this research we demonstrated that Chelex-100 efficiently makes extraction of the DNA from V. inaequalis spores available for direct use inmolecular analyses. Also, the quantity and quality of extracted DNA were shown to be adequate for PCR analysis. Comparatively, the quality of DNA samples isolated using Chelex method was better than those extracted using CTAB. In conclusion, the Chelexmethod is recommended for PCR experiments considering its simplicity and costeffectiveness.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
