Samples from pomegranate trees were collected from cultivations in Khalilabad and Bardaskan (Razavi-Khorassan province, Iran) during summer 2015. The symptomatic plants showed slow decline during one or more years with a reduction in yield and fruit size and yellowing of the leaves, and/or reddening with thickening of veins. Finally the trees showed general dieback. After nucleic acid extraction and PCR/RFLP analyses of 16S ribosomal gene followed by sequencing and phylogenetic analyses a phytoplasma related with ‘Candidatus Phytoplasma pruni’ was identified in the symptomatic samples. The epidemiologic importance of this new phytoplasma-associated in diseased pomegranate in Iran should be further studied to establish control strategies and avoid dissemination of this phytoplasma for the first time reported in the Country.
Karimi, M., Paltrinieri, S., Contaldo, N., Kamali, H., Sajadinejad, M., Ajami, M., et al. (2015). Phytoplasma detection and identification in declining pomegranate in Iran. PHYTOPATHOGENIC MOLLICUTES, 5(2), 95-99 [10.5958/2249-4677.2015.00067].
Phytoplasma detection and identification in declining pomegranate in Iran
PALTRINIERI, SAMANTA;CONTALDO, NICOLETTA;BERTACCINI, ASSUNTA
2015
Abstract
Samples from pomegranate trees were collected from cultivations in Khalilabad and Bardaskan (Razavi-Khorassan province, Iran) during summer 2015. The symptomatic plants showed slow decline during one or more years with a reduction in yield and fruit size and yellowing of the leaves, and/or reddening with thickening of veins. Finally the trees showed general dieback. After nucleic acid extraction and PCR/RFLP analyses of 16S ribosomal gene followed by sequencing and phylogenetic analyses a phytoplasma related with ‘Candidatus Phytoplasma pruni’ was identified in the symptomatic samples. The epidemiologic importance of this new phytoplasma-associated in diseased pomegranate in Iran should be further studied to establish control strategies and avoid dissemination of this phytoplasma for the first time reported in the Country.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.