The effect that pac 1, a yeast-derived ds-RNA specific RNase gene, has on Potato virus X (PVX) and on Potato virus Y (PVY) infections in potato was investigated. Tuber disks of cv. Desirée were transformed with LB4404 Agrobacterium tumefaciens carrying a modified PBI121 binary vector in which the transgene replaced GUS. Trans-formed plantlets were tested for possible acquired resistance by inoculation with purified virus suspensions at different concentrations and were ELISA-tested for virus content 40 days later. Plants of two clones had a significantly lower PVX content (66 to 75% that of the control, respectively), when inoculated with a 1 μg ml-1 suspension of the virus. One of these clones showed also a reduced PVY accumulation (41% that of the control), when inoculated with a 10 μg ml-1 suspension of this virus. Results demonstrated the practical utility of the pac1 gene for countering potato virus infections.

The use of pac 1 gene from Schizosaccaromyces pombe to protect potato from Potato virus Y( PVY) and potato virus X (PVX) infections.

COLALONGO, MARIA CHIARA;CONTALDO, NICOLETTA;FACCIOLI, GIANNI;PAGAN, PAOLO
2005

Abstract

The effect that pac 1, a yeast-derived ds-RNA specific RNase gene, has on Potato virus X (PVX) and on Potato virus Y (PVY) infections in potato was investigated. Tuber disks of cv. Desirée were transformed with LB4404 Agrobacterium tumefaciens carrying a modified PBI121 binary vector in which the transgene replaced GUS. Trans-formed plantlets were tested for possible acquired resistance by inoculation with purified virus suspensions at different concentrations and were ELISA-tested for virus content 40 days later. Plants of two clones had a significantly lower PVX content (66 to 75% that of the control, respectively), when inoculated with a 1 μg ml-1 suspension of the virus. One of these clones showed also a reduced PVY accumulation (41% that of the control), when inoculated with a 10 μg ml-1 suspension of this virus. Results demonstrated the practical utility of the pac1 gene for countering potato virus infections.
C.Colalongo;N.Contaldo;G.Faccioli;P.Pagan
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/5477
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