The effect that pac 1, a yeast-derived ds-RNA specific RNase gene, has on Potato virus X (PVX) and on Potato virus Y (PVY) infections in potato was investigated. Tuber disks of cv. Desirée were transformed with LB4404 Agrobacterium tumefaciens carrying a modified PBI121 binary vector in which the transgene replaced GUS. Trans-formed plantlets were tested for possible acquired resistance by inoculation with purified virus suspensions at different concentrations and were ELISA-tested for virus content 40 days later. Plants of two clones had a significantly lower PVX content (66 to 75% that of the control, respectively), when inoculated with a 1 μg ml-1 suspension of the virus. One of these clones showed also a reduced PVY accumulation (41% that of the control), when inoculated with a 10 μg ml-1 suspension of this virus. Results demonstrated the practical utility of the pac1 gene for countering potato virus infections.
C.Colalongo, N.Contaldo, G.Faccioli, P.Pagan (2005). The use of pac 1 gene from Schizosaccaromyces pombe to protect potato from Potato virus Y( PVY) and potato virus X (PVX) infections. PHYTOPATHOLOGIA MEDITERRANEA, 44(2), 226-231.
The use of pac 1 gene from Schizosaccaromyces pombe to protect potato from Potato virus Y( PVY) and potato virus X (PVX) infections.
COLALONGO, MARIA CHIARA;CONTALDO, NICOLETTA;FACCIOLI, GIANNI;PAGAN, PAOLO
2005
Abstract
The effect that pac 1, a yeast-derived ds-RNA specific RNase gene, has on Potato virus X (PVX) and on Potato virus Y (PVY) infections in potato was investigated. Tuber disks of cv. Desirée were transformed with LB4404 Agrobacterium tumefaciens carrying a modified PBI121 binary vector in which the transgene replaced GUS. Trans-formed plantlets were tested for possible acquired resistance by inoculation with purified virus suspensions at different concentrations and were ELISA-tested for virus content 40 days later. Plants of two clones had a significantly lower PVX content (66 to 75% that of the control, respectively), when inoculated with a 1 μg ml-1 suspension of the virus. One of these clones showed also a reduced PVY accumulation (41% that of the control), when inoculated with a 10 μg ml-1 suspension of this virus. Results demonstrated the practical utility of the pac1 gene for countering potato virus infections.| File | Dimensione | Formato | |
|---|---|---|---|
|
5134-Article Text-5062-1-1-20190716.pdf
accesso aperto
Tipo:
Versione (PDF) editoriale
Licenza:
Licenza per Accesso Aperto. Creative Commons Attribuzione (CCBY)
Dimensione
143.85 kB
Formato
Adobe PDF
|
143.85 kB | Adobe PDF | Visualizza/Apri |
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
