Aim: This preclinical in vivo study aims to evaluate in pigs the influence of the distance of residual periodontal ligament (PDL) and bone on periodontal healing in calibrated surgical defects in Guided Tissue Regeneration (GTR) procedure. Material and Methods: Three adult Sus scrofa domesticus were used in this study. A total of six third mandibular incisors were analysed. After coronectomy of each experimental tooth at crest level a circumferential bone defect was created on five teeth using calibrated piezoelectric inserts maintaining a 2 mm constant width. The depths of the experimental defects were 3, 5, 7, 9 and 11 mm respectively. One tooth served as control. Each defect was accurately root planed and all teeth were submerged using a bio-resorbable bilayer collagen membrane. The mucosal flap was repositioned to achieve a full closure. After 6 months the animals were euthanized, block sections were harvested and maintained at 20°C for micro-CT analysis. Results: Clear signs of root resorption were observed with values directly correlated to the extent of the vertical defect. The medial third of the root was the most impaired. Signs of ankylosis were also recorded in the 3 and 7 mm depth defects. Conclusion: In GTR procedure periodontal ligament and alveolar bone seem to compete for periodontal wound healing and their proximity to the root surface seems to influence the final outcome. The results support the hypothesis of a critical defect dimension for GTR effectiveness.

Aim: This preclinical in vivo study aims to evaluate in pigs the influence of the distance of residual periodontal ligament (PDL) and bone on periodontal healing in calibrated surgical defects in Guided Tissue Regeneration (GTR) procedure. Material and Methods: Three adult Sus scrofa domesticus were used in this study. A total of six third mandibular incisors were analysed. After coronectomy of each experimental tooth at crest level a circumferential bone defect was created on five teeth using calibrated piezoelectric inserts maintaining a 2 mm constant width. The depths of the experimental defects were 3, 5, 7, 9 and 11 mm respectively. One tooth served as control. Each defect was accurately root planed and all teeth were submerged using a bio-resorbable bilayer collagen membrane. The mucosal flap was repositioned to achieve a full closure. After 6 months the animals were euthanized, block sections were harvested and maintained at 20°C for micro-CT analysis. Results: Clear signs of root resorption were observed with values directly correlated to the extent of the vertical defect. The medial third of the root was the most impaired. Signs of ankylosis were also recorded in the 3 and 7 mm depth defects. Conclusion: In GTR procedure periodontal ligament and alveolar bone seem to compete for periodontal wound healing and their proximity to the root surface seems to influence the final outcome. The results support the hypothesis of a critical defect dimension for GTR effectiveness.

Are guided tissue regeneration outcomes influenced by residual periodontal ligament and bone position? An experimental animal model with micro-CT analysis

MONTEVECCHI, MARCO;LO BIANCO, LAURA;MUTTINI, AURELIO;CHECCHI, LUIGI
2015

Abstract

Aim: This preclinical in vivo study aims to evaluate in pigs the influence of the distance of residual periodontal ligament (PDL) and bone on periodontal healing in calibrated surgical defects in Guided Tissue Regeneration (GTR) procedure. Material and Methods: Three adult Sus scrofa domesticus were used in this study. A total of six third mandibular incisors were analysed. After coronectomy of each experimental tooth at crest level a circumferential bone defect was created on five teeth using calibrated piezoelectric inserts maintaining a 2 mm constant width. The depths of the experimental defects were 3, 5, 7, 9 and 11 mm respectively. One tooth served as control. Each defect was accurately root planed and all teeth were submerged using a bio-resorbable bilayer collagen membrane. The mucosal flap was repositioned to achieve a full closure. After 6 months the animals were euthanized, block sections were harvested and maintained at 20°C for micro-CT analysis. Results: Clear signs of root resorption were observed with values directly correlated to the extent of the vertical defect. The medial third of the root was the most impaired. Signs of ankylosis were also recorded in the 3 and 7 mm depth defects. Conclusion: In GTR procedure periodontal ligament and alveolar bone seem to compete for periodontal wound healing and their proximity to the root surface seems to influence the final outcome. The results support the hypothesis of a critical defect dimension for GTR effectiveness.
Montevecchi, Marco; Lo Bianco, Laura; Parilli, Anna Paola; Muttini, Aurelio; Checchi, Luigi
File in questo prodotto:
Eventuali allegati, non sono esposti

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/546318
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact