The aim of this study was to verify the effects of vitrification on in vitro produced bovine blastocysts. Day 7 blastocysts and expanded blastocysts were divided in two groups: vitrified embryos (V); control group (C). Cryopreserved embryos were thawed on the same day and cultured for 48 h to asses the re-expansion and hatching. Expanded blastocysts were stained with Propidium Iodide and Hoechts 33258. The number of live and total cells was similar in both groups (58.65±13.64 vs 63.92±26.66; 79.50±21.37 vs 74.60±28.36); the number of dead cells and the proportion of expanded blastocysts were significantly different (20.46±11.58 vs 10.68±10.24; 50.94% vs 68.25%).
Vitrificazione di blastocisti bovine prodotte in vitro / Merlo B.; Iacono E.; Prati F.; Mari G.. - ELETTRONICO. - LXI:(2007), pp. 31-32. (Intervento presentato al convegno Congresso Annuale della Società Italiana delle Scienze Veterinarie tenutosi a Salsomaggiore Terme nel 26-29 Settembre 2007).
Vitrificazione di blastocisti bovine prodotte in vitro
MERLO, BARBARA;IACONO, ELEONORA;PRATI, FRANCESCA;MARI, GAETANO
2007
Abstract
The aim of this study was to verify the effects of vitrification on in vitro produced bovine blastocysts. Day 7 blastocysts and expanded blastocysts were divided in two groups: vitrified embryos (V); control group (C). Cryopreserved embryos were thawed on the same day and cultured for 48 h to asses the re-expansion and hatching. Expanded blastocysts were stained with Propidium Iodide and Hoechts 33258. The number of live and total cells was similar in both groups (58.65±13.64 vs 63.92±26.66; 79.50±21.37 vs 74.60±28.36); the number of dead cells and the proportion of expanded blastocysts were significantly different (20.46±11.58 vs 10.68±10.24; 50.94% vs 68.25%).I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
