The aim of this study was to verify the effects of vitrification on in vitro produced bovine blastocysts. Day 7 blastocysts and expanded blastocysts were divided in two groups: vitrified embryos (V); control group (C). Cryopreserved embryos were thawed on the same day and cultured for 48 h to asses the re-expansion and hatching. Expanded blastocysts were stained with Propidium Iodide and Hoechts 33258. The number of live and total cells was similar in both groups (58.65±13.64 vs 63.92±26.66; 79.50±21.37 vs 74.60±28.36); the number of dead cells and the proportion of expanded blastocysts were significantly different (20.46±11.58 vs 10.68±10.24; 50.94% vs 68.25%).
Merlo B., Iacono E., Prati F., Mari G. (2007). Vitrificazione di blastocisti bovine prodotte in vitro. s.l : s.n.
Vitrificazione di blastocisti bovine prodotte in vitro
MERLO, BARBARA;IACONO, ELEONORA;PRATI, FRANCESCA;MARI, GAETANO
2007
Abstract
The aim of this study was to verify the effects of vitrification on in vitro produced bovine blastocysts. Day 7 blastocysts and expanded blastocysts were divided in two groups: vitrified embryos (V); control group (C). Cryopreserved embryos were thawed on the same day and cultured for 48 h to asses the re-expansion and hatching. Expanded blastocysts were stained with Propidium Iodide and Hoechts 33258. The number of live and total cells was similar in both groups (58.65±13.64 vs 63.92±26.66; 79.50±21.37 vs 74.60±28.36); the number of dead cells and the proportion of expanded blastocysts were significantly different (20.46±11.58 vs 10.68±10.24; 50.94% vs 68.25%).I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
