During 2010-2013, samples from 267 apple trees growing in six regions of Poland were tested for phy- toplasma presence. ‘Candidatus Phytoplasma mali’ was detected in 17 samples from phloem tissue of apple shoots showing typical apple proliferation symptoms. Molecular characterization of these strains was conducted using PCR-RFLP and sequence analyses of three regions: ribosomal DNA, ribosomal protein and non-ribosomal DNA fragment including nitroreductase and rhodanese-like protein genes. Fragments of 16S rDNA plus 16S-23S spacer region from the ‘Ca. P. mali’ strains showed two restriction profiles: P-I and P-II when digested with HpaII enzyme. Moreover, based on results of nitroreductase and rhodanese-like protein genes, these phytoplasmas were grouped into two subtypes: AP-15 and AT-1. The majority of the ‘Ca. P. mali’ strains was classified to the AP-15 subtype. Two strains were grouped to the AT-1 subtype and another showed both AP-15 and AT-1 profiles. Based on results of analysis of rpl22 and rps3 ribosomal protein genes, most of the ‘Ca. P. mali’ strains were identified as belonging to rpX-A. Only one strain was affiliated to rpX-B subgroup.
Genetic diversity of 'Candidatus Phytoplasma mali' strains in Poland
BERTACCINI, ASSUNTA
2015
Abstract
During 2010-2013, samples from 267 apple trees growing in six regions of Poland were tested for phy- toplasma presence. ‘Candidatus Phytoplasma mali’ was detected in 17 samples from phloem tissue of apple shoots showing typical apple proliferation symptoms. Molecular characterization of these strains was conducted using PCR-RFLP and sequence analyses of three regions: ribosomal DNA, ribosomal protein and non-ribosomal DNA fragment including nitroreductase and rhodanese-like protein genes. Fragments of 16S rDNA plus 16S-23S spacer region from the ‘Ca. P. mali’ strains showed two restriction profiles: P-I and P-II when digested with HpaII enzyme. Moreover, based on results of nitroreductase and rhodanese-like protein genes, these phytoplasmas were grouped into two subtypes: AP-15 and AT-1. The majority of the ‘Ca. P. mali’ strains was classified to the AP-15 subtype. Two strains were grouped to the AT-1 subtype and another showed both AP-15 and AT-1 profiles. Based on results of analysis of rpl22 and rps3 ribosomal protein genes, most of the ‘Ca. P. mali’ strains were identified as belonging to rpX-A. Only one strain was affiliated to rpX-B subgroup.File | Dimensione | Formato | |
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