During 2006 and 2007 eight batches of seed potatoes collected in different locations, and belonging to one cultivar were planted in spring under greenhouse conditions and tested after 2 months to verify phytoplasma presence. A total of 635 asymptomatic plantlets were examined. Nucleic acid was extracted from small shoots from either a single plant or from batches of 3 plants each. Nested PCR on both single and grouped samples with general ribosomal primers, without spacer region allowed specific phytoplasma detection. Phytoplasmas belonging to diverse ribosomal groups were identified after RFLP analyses according to the batch tested. Ribosomal subgroups 16SrI-B (related to ‘Candidatus phytoplasma asteris’), 16SrI-C (related to clover phyllody: CPh), 16SrII-D (related to tomato big bud from Australia: TBB), 16SrX-A (related to ‘Ca. P. mali’), and 16SrXII-A (related to stolbur) were identified in different percentages. After further validation tests, the system can be used to screen high quality seed potatos for phytoplasmas.
Detection of phytoplasmas in plantlets grown from different batches of seed-potatoes / Paltrinieri S.; A. Bertaccini.. - In: BULLETIN OF INSECTOLOGY. - ISSN 1721-8861. - STAMPA. - 60:(2007), pp. 379-380. (Intervento presentato al convegno First IPWG Meeting tenutosi a Bologna nel 12-15 November 2007).
Detection of phytoplasmas in plantlets grown from different batches of seed-potatoes.
PALTRINIERI, SAMANTA;BERTACCINI, ASSUNTA
2007
Abstract
During 2006 and 2007 eight batches of seed potatoes collected in different locations, and belonging to one cultivar were planted in spring under greenhouse conditions and tested after 2 months to verify phytoplasma presence. A total of 635 asymptomatic plantlets were examined. Nucleic acid was extracted from small shoots from either a single plant or from batches of 3 plants each. Nested PCR on both single and grouped samples with general ribosomal primers, without spacer region allowed specific phytoplasma detection. Phytoplasmas belonging to diverse ribosomal groups were identified after RFLP analyses according to the batch tested. Ribosomal subgroups 16SrI-B (related to ‘Candidatus phytoplasma asteris’), 16SrI-C (related to clover phyllody: CPh), 16SrII-D (related to tomato big bud from Australia: TBB), 16SrX-A (related to ‘Ca. P. mali’), and 16SrXII-A (related to stolbur) were identified in different percentages. After further validation tests, the system can be used to screen high quality seed potatos for phytoplasmas.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
