Extensive survey to detect and identify phytoplasmas associated with Chilean grapevines was performed from 2003 to 2005 sampling symptomatic or asymptomatic grapevine plants. Nested PCR amplification allow the detection of phytoplasmas in 28 out of 90 samples. RFLP analyses identified phytoplasmas as belonging to ribosomal subgroups 16SrI-B, 16SrI-C, 16SrVII-A and 16SrXII-A. Cloning and sequencing of selected strains allow to detect genetic variability in 16SrXII-A strains.
Fiore N., S. Prodan, S. Paltrinieri, A. Gajardo, S. Botti, A.M. Pino, et al. (2007). Molecular characterization of phytoplasmas in Chilean grapevines..
Molecular characterization of phytoplasmas in Chilean grapevines.
PALTRINIERI, SAMANTA;BOTTI, SIMONA;BERTACCINI, ASSUNTA
2007
Abstract
Extensive survey to detect and identify phytoplasmas associated with Chilean grapevines was performed from 2003 to 2005 sampling symptomatic or asymptomatic grapevine plants. Nested PCR amplification allow the detection of phytoplasmas in 28 out of 90 samples. RFLP analyses identified phytoplasmas as belonging to ribosomal subgroups 16SrI-B, 16SrI-C, 16SrVII-A and 16SrXII-A. Cloning and sequencing of selected strains allow to detect genetic variability in 16SrXII-A strains.File in questo prodotto:
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