Cactus pear plants showing proliferation and stunting of cladodes in Californian cultivations were tested in order to define a molecular methodology for reliable phytoplasma detection. After several unsuccessful trials a simple extraction method was developed to reduce the mucilage content in nucleic acid preparations that was seriously affecting pathogen detection. Nested PCR on 16S ribosomal gene and RFLP analyses together with sequencing of obtained amplicons allow to verify the presence in symptomatic plants of 16SrV-A and 16SrI-B phytoplasmas respectively related to ‘Candidatus Phytoplasma ulmi’ and ‘Ca. P. asteris’.
Bertaccini A., A. Calari, P. Felker. (2007). Developing a method for phytoplasma identification in cactus pear samples from California..
Developing a method for phytoplasma identification in cactus pear samples from California.
BERTACCINI, ASSUNTA;CALARI, ALBERTO;
2007
Abstract
Cactus pear plants showing proliferation and stunting of cladodes in Californian cultivations were tested in order to define a molecular methodology for reliable phytoplasma detection. After several unsuccessful trials a simple extraction method was developed to reduce the mucilage content in nucleic acid preparations that was seriously affecting pathogen detection. Nested PCR on 16S ribosomal gene and RFLP analyses together with sequencing of obtained amplicons allow to verify the presence in symptomatic plants of 16SrV-A and 16SrI-B phytoplasmas respectively related to ‘Candidatus Phytoplasma ulmi’ and ‘Ca. P. asteris’.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
