“Flavescence dorée” (FD) and “bois noir” (BN) are two grapevine diseases threatening many wine producing countries. Routine detection of FD and BN phytoplasmas mainly rely on nested PCR/RFLP analyses which are time and labour consuming. Realtime PCR can be employed to procedure speed up by coupling specificity of primers and probes and sensitivity of detection system. In this paper a real-time PCR based procedure to detect FD and BN phytoplasmas, suitable for large number of sample testing is described.
Establishing a real-time PCR detection procedure of ”flavescence dorée” and “bois noir” phytoplasmas for mass screening / Gori M.; R. Monnanni; M. Buiatti; E. Goti; S. Carnevale; L. Da Prato; A. Bertaccini; S. Biricolti. - In: BULLETIN OF INSECTOLOGY. - ISSN 1721-8861. - STAMPA. - 60:(2007), pp. 255-256. (Intervento presentato al convegno First IPWG Meeting tenutosi a Bologna nel 12-15 November 2007).
Establishing a real-time PCR detection procedure of ”flavescence dorée” and “bois noir” phytoplasmas for mass screening.
BERTACCINI, ASSUNTA;
2007
Abstract
“Flavescence dorée” (FD) and “bois noir” (BN) are two grapevine diseases threatening many wine producing countries. Routine detection of FD and BN phytoplasmas mainly rely on nested PCR/RFLP analyses which are time and labour consuming. Realtime PCR can be employed to procedure speed up by coupling specificity of primers and probes and sensitivity of detection system. In this paper a real-time PCR based procedure to detect FD and BN phytoplasmas, suitable for large number of sample testing is described.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
