Objectives: Platelet Rich Plasma (PRP) is a biological compound rich of growth factors able to support healing processes, and it represents a fascinating tool for clinicians. However results on its potentiality as intra-articular treatment for cartilage degenerative lesions in osteoarthritis (OA) are still controversial, due to the lack of well designed studies and the differences in the preparation procedures. The aim of this study was to analyze the modifications induced by two different PRP preparations on chondrocytes and sinoviocytes in culture, by documenting changes in proliferations, gene expression, and extracellular matrix components deposition. Methods: Blood collected from 10 volunteers was used to obtain two PRP preparations derived respectively from a ‘‘one-spinning procedure’’, to obtain a pure platelet concentrate without leukocytes but with a limited number of platelets (P-PRP), and by a ‘‘two-spinning approach’’, producing a product characterized by a higher platelet concentration and the presence of leukocytes (L-PRP). Results: Our data showed that L-PRP contains higher levels of growth factors and cytokines compared to P-PRP. Both PRPs stimulate chondrocytes proliferation during all the culture period evaluated; a significant enhancement in cell growth was induced by P-PRP respect to L-PRP only at day 7. Moreover, P-PRP stimulates chondrocyte anabolism as demonstrated by the expression of collagen type II and aggrecan, while L-PRP promotes catabolic pathways in which some different cytokines are involved. Interesting, L-PRP induces a higher expression of hyaluronic acid synthase-2 and hyaluronan compared to P-PRP. On sinoviocytes, L-PRP is able to sustain the up-regulation of proinflammatory factors, such as IL-1beta, IL-8 and FGF-2, together with a down modulation of HGF and TIMP-4 expression, two factors that have been recognized as anti-catabolic mediators in cartilage, whereas P-PRP was not able to exert a differential pattern of biological effect compared to PPP. Conclusions: Our study highlights that the two PRPs induce distinct effects on cultured human chondrocytes and sinoviocytes. This is due to the different content in PRP of platelets, leukocytes, growth factors and molecules that act synergically to regulate several biological processes which could influence the regeneration of a functional tissue.
Prp with or without leukocytes: any difference on chondrocytes and synoviocytes culture?
DI MATTEO, BERARDO;MARIANI, ERMINIA;FILARDO, GIUSEPPE;KON, ELIZAVETA;ROFFI, ALICE;GRIGOLO, BRUNELLA;MARCACCI, MAURILIO
2014
Abstract
Objectives: Platelet Rich Plasma (PRP) is a biological compound rich of growth factors able to support healing processes, and it represents a fascinating tool for clinicians. However results on its potentiality as intra-articular treatment for cartilage degenerative lesions in osteoarthritis (OA) are still controversial, due to the lack of well designed studies and the differences in the preparation procedures. The aim of this study was to analyze the modifications induced by two different PRP preparations on chondrocytes and sinoviocytes in culture, by documenting changes in proliferations, gene expression, and extracellular matrix components deposition. Methods: Blood collected from 10 volunteers was used to obtain two PRP preparations derived respectively from a ‘‘one-spinning procedure’’, to obtain a pure platelet concentrate without leukocytes but with a limited number of platelets (P-PRP), and by a ‘‘two-spinning approach’’, producing a product characterized by a higher platelet concentration and the presence of leukocytes (L-PRP). Results: Our data showed that L-PRP contains higher levels of growth factors and cytokines compared to P-PRP. Both PRPs stimulate chondrocytes proliferation during all the culture period evaluated; a significant enhancement in cell growth was induced by P-PRP respect to L-PRP only at day 7. Moreover, P-PRP stimulates chondrocyte anabolism as demonstrated by the expression of collagen type II and aggrecan, while L-PRP promotes catabolic pathways in which some different cytokines are involved. Interesting, L-PRP induces a higher expression of hyaluronic acid synthase-2 and hyaluronan compared to P-PRP. On sinoviocytes, L-PRP is able to sustain the up-regulation of proinflammatory factors, such as IL-1beta, IL-8 and FGF-2, together with a down modulation of HGF and TIMP-4 expression, two factors that have been recognized as anti-catabolic mediators in cartilage, whereas P-PRP was not able to exert a differential pattern of biological effect compared to PPP. Conclusions: Our study highlights that the two PRPs induce distinct effects on cultured human chondrocytes and sinoviocytes. This is due to the different content in PRP of platelets, leukocytes, growth factors and molecules that act synergically to regulate several biological processes which could influence the regeneration of a functional tissue.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
