We tested the hypothesis that cardiac regeneration through local delivery of adipose-derived stem cells (ASCs), activation of resident cardiac stem cells via growth factors (GFs) [hepatocyte growth factor (HGF) and insulin-like growth factor 1 (IGF-1):GFs] or both, are improved by pharmacologically active microcarriers (PAMs) interacting with cells/molecules conveyed on their surface. Rats with one-month old myocardial infarction were treated with ASCs, ASCs+PAMs, GF-releasing PAMs, ASCs+GF-releasing PAMs or vehicle. Two weeks later, hemodynamic function and inducibility of ventricular arrhythmias (VAs) were assessed. Eventually, the hearts were subjected to anatomical and immunohistochemical analyses. A significant ASCs engraftment and the largest improvement in cardiac mechanics occurred in ASC+GF-releasing PAM rats which by contrast were more vulnerable to VAs. Thus, PAMs may improve cell/GF-based cardiac regeneration although caution should be paid on the electrophysiological impact of their physical interaction with the myocardium.

Cardiac regeneration by pharmacologically active microcarriers releasing growth factors and/or transporting adipose-derived stem cells / Savi, Monia; Bocchi, Leonardo; Fiumana, Emanuela; Frati, Caterina; Bonafé, Francesca; Cavalli, Stefano; Morselli, Paolo Giovanni; Karam, Jean-Pierre; Montero-Menei, Claudia; Caldarera, Claudio Marcello; Guarnieri, Carlo; Muscari, Claudio; Stilli, Donatella; Quaini, Federico; Musso, Ezio. - In: JOURNAL OF BIOLOGICAL RESEARCH. - ISSN 1826-8838. - ELETTRONICO. - 87:1(2014), pp. 2141.46-2141.47. (Intervento presentato al convegno 85° CONVEGNO SIBS - LA BIOLOGIA SPERIMENTALE: DALLE MOLECOLE ALL’ORGANISMO tenutosi a Parma nel 29 - 30 novembre 2012) [10.4081/jbr.2014.2141].

Cardiac regeneration by pharmacologically active microcarriers releasing growth factors and/or transporting adipose-derived stem cells

FIUMANA, EMANUELA;BONAFÈ, FRANCESCA;MORSELLI, PAOLO;CALDARERA, CLAUDIO MARCELLO;GUARNIERI, CARLO;MUSCARI, CLAUDIO;
2014

Abstract

We tested the hypothesis that cardiac regeneration through local delivery of adipose-derived stem cells (ASCs), activation of resident cardiac stem cells via growth factors (GFs) [hepatocyte growth factor (HGF) and insulin-like growth factor 1 (IGF-1):GFs] or both, are improved by pharmacologically active microcarriers (PAMs) interacting with cells/molecules conveyed on their surface. Rats with one-month old myocardial infarction were treated with ASCs, ASCs+PAMs, GF-releasing PAMs, ASCs+GF-releasing PAMs or vehicle. Two weeks later, hemodynamic function and inducibility of ventricular arrhythmias (VAs) were assessed. Eventually, the hearts were subjected to anatomical and immunohistochemical analyses. A significant ASCs engraftment and the largest improvement in cardiac mechanics occurred in ASC+GF-releasing PAM rats which by contrast were more vulnerable to VAs. Thus, PAMs may improve cell/GF-based cardiac regeneration although caution should be paid on the electrophysiological impact of their physical interaction with the myocardium.
2014
Proceedings of the 85th SIBS Congress
46
47
Cardiac regeneration by pharmacologically active microcarriers releasing growth factors and/or transporting adipose-derived stem cells / Savi, Monia; Bocchi, Leonardo; Fiumana, Emanuela; Frati, Caterina; Bonafé, Francesca; Cavalli, Stefano; Morselli, Paolo Giovanni; Karam, Jean-Pierre; Montero-Menei, Claudia; Caldarera, Claudio Marcello; Guarnieri, Carlo; Muscari, Claudio; Stilli, Donatella; Quaini, Federico; Musso, Ezio. - In: JOURNAL OF BIOLOGICAL RESEARCH. - ISSN 1826-8838. - ELETTRONICO. - 87:1(2014), pp. 2141.46-2141.47. (Intervento presentato al convegno 85° CONVEGNO SIBS - LA BIOLOGIA SPERIMENTALE: DALLE MOLECOLE ALL’ORGANISMO tenutosi a Parma nel 29 - 30 novembre 2012) [10.4081/jbr.2014.2141].
Savi, Monia; Bocchi, Leonardo; Fiumana, Emanuela; Frati, Caterina; Bonafé, Francesca; Cavalli, Stefano; Morselli, Paolo Giovanni; Karam, Jean-Pierre; Montero-Menei, Claudia; Caldarera, Claudio Marcello; Guarnieri, Carlo; Muscari, Claudio; Stilli, Donatella; Quaini, Federico; Musso, Ezio
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/518969
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