Root and crown rot of wheat, caused by Fusarium culmorum (Fc), is a serious disease worldwide, particularly in Iran. Currently, the mechanisms underlying resistance to Fusarium-caused diseases are still unknown. Methyl jasmonate (MeJA) has been identified as a vital cellular regulator, and the effect of exogenous MeJA application during wheat-Fc interaction has not been studied previously at the molecular level. This study, using real-time quantitative PCR, was carried out to determine the expression of seven host defense-associated genes consisting of PR3, PR4, PR5, TaPERO, LOX, PAL and cytochrome P450 gene (CYP709C1) at 48, 72 and 96 h after Fc-inoculation (hai) and to investigate the relationship between the induced resistance by MeJA application and the gene expression patterns. The results showed that several genes were induced earlier (48 hai) and to higher levels in the resistant cv. Sumai3 than in susceptible cv. Falat. Many Fc-induced genes were also activated and induced by MeJA particularly during the later stages of infection; however, in contrast to defense-gene induction by the pathogen, there was not a general trend of higher induction in Sumai3 compared to Falat following this chemical treatment. The chemically induced protection significantly reduced the development of necrotic symptoms in both cultivars over a 3-week period after inoculation; however, this reduction was greater in Falat than in Sumai3 relative to the controls. Surprisingly, the expression of these genes was also expressed in crown tissue that had not yet been in contact with the fungus, signifying that a form of systemic response was taking place in this interaction. This is the first work reporting the effect of timing application of MeJA during Fc infection and comparing the defense-related gene expression between root and crown tissues of wheat genotypes. Moreover, soil-drench application of MeJA resulted in strong induction, verifying the success of this application method in systemically activating JA signaling for the first time. These results provided important intimations for designing strategies to curtail diseases caused by Fusarium.

Methyl Jasmonate Strengthens Wheat Plants Against Root and Crown Rot Pathogen Fusarium culmorum Infection / Motallebi P; Niknam V; Ebrahimzadeh H; Hashemi, M; Pisi A; Prodi A; Tonti, S; Nipoti P. - In: JOURNAL OF PLANT GROWTH REGULATION. - ISSN 0721-7595. - ELETTRONICO. - 34:3(2015), pp. 624-636. [10.1007/s00344-015-9496-7]

Methyl Jasmonate Strengthens Wheat Plants Against Root and Crown Rot Pathogen Fusarium culmorum Infection

MOTALLEBI, PARASTOO;PISI, ANNAMARIA;PRODI, ANTONIO;TONTI, STEFANO;NIPOTI, PAOLA
2015

Abstract

Root and crown rot of wheat, caused by Fusarium culmorum (Fc), is a serious disease worldwide, particularly in Iran. Currently, the mechanisms underlying resistance to Fusarium-caused diseases are still unknown. Methyl jasmonate (MeJA) has been identified as a vital cellular regulator, and the effect of exogenous MeJA application during wheat-Fc interaction has not been studied previously at the molecular level. This study, using real-time quantitative PCR, was carried out to determine the expression of seven host defense-associated genes consisting of PR3, PR4, PR5, TaPERO, LOX, PAL and cytochrome P450 gene (CYP709C1) at 48, 72 and 96 h after Fc-inoculation (hai) and to investigate the relationship between the induced resistance by MeJA application and the gene expression patterns. The results showed that several genes were induced earlier (48 hai) and to higher levels in the resistant cv. Sumai3 than in susceptible cv. Falat. Many Fc-induced genes were also activated and induced by MeJA particularly during the later stages of infection; however, in contrast to defense-gene induction by the pathogen, there was not a general trend of higher induction in Sumai3 compared to Falat following this chemical treatment. The chemically induced protection significantly reduced the development of necrotic symptoms in both cultivars over a 3-week period after inoculation; however, this reduction was greater in Falat than in Sumai3 relative to the controls. Surprisingly, the expression of these genes was also expressed in crown tissue that had not yet been in contact with the fungus, signifying that a form of systemic response was taking place in this interaction. This is the first work reporting the effect of timing application of MeJA during Fc infection and comparing the defense-related gene expression between root and crown tissues of wheat genotypes. Moreover, soil-drench application of MeJA resulted in strong induction, verifying the success of this application method in systemically activating JA signaling for the first time. These results provided important intimations for designing strategies to curtail diseases caused by Fusarium.
2015
Methyl Jasmonate Strengthens Wheat Plants Against Root and Crown Rot Pathogen Fusarium culmorum Infection / Motallebi P; Niknam V; Ebrahimzadeh H; Hashemi, M; Pisi A; Prodi A; Tonti, S; Nipoti P. - In: JOURNAL OF PLANT GROWTH REGULATION. - ISSN 0721-7595. - ELETTRONICO. - 34:3(2015), pp. 624-636. [10.1007/s00344-015-9496-7]
Motallebi P; Niknam V; Ebrahimzadeh H; Hashemi, M; Pisi A; Prodi A; Tonti, S; Nipoti P
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/512790
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