Study of the intestinal microflora - Intestinal flora: methods of analysis - (Module 4 - advanced)

Topic I: “ Classical approaches” discusses about the classical methodology of isolation and identification of intestinal microbiota. The topic analyse the possibility to identify genera and the species with morphological and physiological tools. Topic II. “ Molecular methods” provides basic knowledge for the application of the recentness techniques for the attribution of microorganism at specific groups based on gene expression, gene ibridation and DNA amplification and sequencing. In Topic III “ Little discussion about molecular techniques” the main molecular methods are compared in their usefulness in identification and taxonomical collocation. The importance of having standard reference strain give importance to Topic IV “ Bacterial preservation”. It provides knowledge about the possibility to utilize long term methods for conservation of specific strains. Topic V “Genus Bifidobacterium”, Topic VI “Genus Bacteroides”, Topic VII “Genus Eubacterium”, Topic VIII “Genus Lactobacillus”, gives detailed information about the methods specific for the genus.