A method has been developed for the quantitation of the antiepileptic drug vigabatrin in human plasma. It is based on capillary electrophoresis with laser-induced fluorescence (LIF) detection. The effect of the pH of the buffer and of N-methylglucamine addition to the background electrolyte constituent was investigated. The final background electrolyte consisted of 50 mM borate buffer, pH 9.0, with 100 mM N-methylglucamine and enabled separation within 12 min at 20 kV voltage. A solid phase extraction procedure was used for pre-treatment of biological samples, based on mixed-mode lipophilic – cation exchange cartridges, followed by a derivatisation step with 6-carboxyfluorescein-N-succinimidyl ester. Fluorescence was excited by an Ar-Ion laser (excitation wavelength = 488 nm). Linearity was observed in the 10-120 ng mL-1 plasma concentration range. Extraction yield was >96%, precision (expressed as RSD) was <6.7%, accuracy (recovery) was between 97.0 and 101.6%. The method has been successfully applied to the analysis of vigabatrin in plasma of epileptic patients undergoing therapy with the drug.

Determination of vigabatrin in human plasma by means of capillary electrophoresis with laser-induced fluorescence detection

MUSENGA, ALESSANDRO;MANDRIOLI, ROBERTO;RAGGI, MARIA AUGUSTA
2007

Abstract

A method has been developed for the quantitation of the antiepileptic drug vigabatrin in human plasma. It is based on capillary electrophoresis with laser-induced fluorescence (LIF) detection. The effect of the pH of the buffer and of N-methylglucamine addition to the background electrolyte constituent was investigated. The final background electrolyte consisted of 50 mM borate buffer, pH 9.0, with 100 mM N-methylglucamine and enabled separation within 12 min at 20 kV voltage. A solid phase extraction procedure was used for pre-treatment of biological samples, based on mixed-mode lipophilic – cation exchange cartridges, followed by a derivatisation step with 6-carboxyfluorescein-N-succinimidyl ester. Fluorescence was excited by an Ar-Ion laser (excitation wavelength = 488 nm). Linearity was observed in the 10-120 ng mL-1 plasma concentration range. Extraction yield was >96%, precision (expressed as RSD) was <6.7%, accuracy (recovery) was between 97.0 and 101.6%. The method has been successfully applied to the analysis of vigabatrin in plasma of epileptic patients undergoing therapy with the drug.
2007
A. Musenga; R. Mandrioli; I. Comin; E. Kenndler; M.A. Raggi
File in questo prodotto:
Eventuali allegati, non sono esposti

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/50086
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? 0
  • Scopus 18
  • ???jsp.display-item.citation.isi??? 18
social impact