Pulsed Electromagnetic Fields (PEMFs) are commonly used in the clinical practice to treat several pathologies like osteotomies, avascular necrosis of the femoral head and bone grafts. The sequences of events by which electromagnetic stimulation can lead its effects on bone healing are not completely understood. In order to get more information on the mechanism of action of PEMFs, we asked whether a) PEMFs are able to induce changes in the production of growth factors in human osteoblasts and in human mesenchymal stem cells b) the production of growth factors induced by PEMFs vary at different time points (12 and 24 hrs) c) PEMFs induce a different production of growth factors in cells at different stages of differentiation like human Mesenchymal Stem cells and human osteoblasts. Twenty seven genes out of the 84 studied were significantly over and down expressed and fourteen of those genes up or down regulated are related to bone formation or inflammation. Our data demonstrate that PEMFs exert their effects by activating or suppressing a large number of growth factors simultaneously with different functions and that this variation of expression varies depending on time and depending on the different stage of differentiation of the cell.
EARLY EFFECTS OF PULSED ELECTROMAGNETIC FIELDS ON HUMAN OSTEOBLASTS AND MESENCHYMAL STEM CELLS
SCAPOLI, LUCA;PALMIERI, ANNALISA;PEZZETTI, FURIO;
2011
Abstract
Pulsed Electromagnetic Fields (PEMFs) are commonly used in the clinical practice to treat several pathologies like osteotomies, avascular necrosis of the femoral head and bone grafts. The sequences of events by which electromagnetic stimulation can lead its effects on bone healing are not completely understood. In order to get more information on the mechanism of action of PEMFs, we asked whether a) PEMFs are able to induce changes in the production of growth factors in human osteoblasts and in human mesenchymal stem cells b) the production of growth factors induced by PEMFs vary at different time points (12 and 24 hrs) c) PEMFs induce a different production of growth factors in cells at different stages of differentiation like human Mesenchymal Stem cells and human osteoblasts. Twenty seven genes out of the 84 studied were significantly over and down expressed and fourteen of those genes up or down regulated are related to bone formation or inflammation. Our data demonstrate that PEMFs exert their effects by activating or suppressing a large number of growth factors simultaneously with different functions and that this variation of expression varies depending on time and depending on the different stage of differentiation of the cell.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
