The purification of antibodies is conventionally performed using affinity chromatography columns, with Protein A as ligand. The development of valid alternatives to Protein A is one of the challenges of the research in downstream processing, which becomes more important as the production capability of the biopharmaceutical industry increases. The objective of this work is the characterization of affinity membranes derivatized with two different synthetic ligands that show high specificity for immunoglobulins. The affinity membranes have been prepared and characterized, in view of their application in the capture purification step.
Boi, C., Sarti, G.C. (2007). Development and Characterization of Affinity Membranes for Immunoglobulin Purification. SEPARATION SCIENCE AND TECHNOLOGY, 42, 2987-3001 [10.1080/01496390701560140].
Development and Characterization of Affinity Membranes for Immunoglobulin Purification
BOI, CRISTIANA;SARTI, GIULIO CESARE
2007
Abstract
The purification of antibodies is conventionally performed using affinity chromatography columns, with Protein A as ligand. The development of valid alternatives to Protein A is one of the challenges of the research in downstream processing, which becomes more important as the production capability of the biopharmaceutical industry increases. The objective of this work is the characterization of affinity membranes derivatized with two different synthetic ligands that show high specificity for immunoglobulins. The affinity membranes have been prepared and characterized, in view of their application in the capture purification step.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
