A region of glucokinase (GCK) gene was sequenced in 14 horses of 14 different breeds. The resulting GCK nucleotide sequence (GenBank number EF136885) showed 77% homology with human GCK gene portion containing the upstream promoter region and the corresponding 5’ UTR of the exon 1. Conserved regulatory sequences near the putative transcriptional start site were identified. The obtained sequences were aligned to detect polymorphism. A new C>T transition within the 5’ UTR of exon 1 was found. Allele frequencies of this polymorphism were studied by PCR-RFLP in 193 horses of 14 breeds (Bardigiano, 21; Esperia Pony, 5; Haflinger, 10; Italian Heavy Draught Horse, 28; Italian Saddle, 25; Italian Trotter, 16; Lipizzan, 12; Maremmano, 15; Murgese, 14; Norico, 10; Salernitano, 12; Thoroughbred, 10; Tolfetano, 7 and Ventasso Horse, 8). The polymorphism was found in all breeds and differences in allelic frequencies among the breeds were observed. The new SNP identified within a regulative region of GCK gene, which plays an important role in insulin secretion and feeding behaviour, could be used for association studies with performance traits of the horses

Analysis of upstream promoter region and corresponding 5'UTR of glucokinase (GCK) gene in horse breeds / S. Dall'Olio; L. Minieri. - In: ITALIAN JOURNAL OF ANIMAL SCIENCE. - ISSN 1594-4077. - STAMPA. - 6 (Supplement 1):(2007), pp. 106-108. (Intervento presentato al convegno The ASPA 17th Congress tenutosi a Alghero nel May 29 - June 1, 2007).

Analysis of upstream promoter region and corresponding 5'UTR of glucokinase (GCK) gene in horse breeds

DALL'OLIO, STEFANIA;
2007

Abstract

A region of glucokinase (GCK) gene was sequenced in 14 horses of 14 different breeds. The resulting GCK nucleotide sequence (GenBank number EF136885) showed 77% homology with human GCK gene portion containing the upstream promoter region and the corresponding 5’ UTR of the exon 1. Conserved regulatory sequences near the putative transcriptional start site were identified. The obtained sequences were aligned to detect polymorphism. A new C>T transition within the 5’ UTR of exon 1 was found. Allele frequencies of this polymorphism were studied by PCR-RFLP in 193 horses of 14 breeds (Bardigiano, 21; Esperia Pony, 5; Haflinger, 10; Italian Heavy Draught Horse, 28; Italian Saddle, 25; Italian Trotter, 16; Lipizzan, 12; Maremmano, 15; Murgese, 14; Norico, 10; Salernitano, 12; Thoroughbred, 10; Tolfetano, 7 and Ventasso Horse, 8). The polymorphism was found in all breeds and differences in allelic frequencies among the breeds were observed. The new SNP identified within a regulative region of GCK gene, which plays an important role in insulin secretion and feeding behaviour, could be used for association studies with performance traits of the horses
2007
106
108
Analysis of upstream promoter region and corresponding 5'UTR of glucokinase (GCK) gene in horse breeds / S. Dall'Olio; L. Minieri. - In: ITALIAN JOURNAL OF ANIMAL SCIENCE. - ISSN 1594-4077. - STAMPA. - 6 (Supplement 1):(2007), pp. 106-108. (Intervento presentato al convegno The ASPA 17th Congress tenutosi a Alghero nel May 29 - June 1, 2007).
S. Dall'Olio; L. Minieri
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/49217
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