From the caudices of the Passifloraceae Adenia lanceolata and Adenia stenodactyla, two lectins called lanceolin and stenodactylin, respectively, were purified by affinity chromatography on CL Sepharose 6B. The lectins are glycoproteins with Mr 61,243 (lanceolin) and 63,131 stenodactylin), consisting of an enzymatic A chain linked to a larger B chain with lectin properties, with N-terminal amino acid sequences similar to that of volkensin, the toxic lectin from Adenia volkensii. The lectins agglutinate red blood cells, inhibit protein synthesis both by a cell-free system and by whole cells, and depurinate ribosomes and DNA, but not tRNA or poly(A). They are highly toxic to cells, in which they induce apoptosis, and to mice, with LD50’s 8.16 μg/kg (lanceolin), and 2.76 μg/kg (stenodactylin) at 48 h. Thus lanceolin and stenodactylin have all the properties of the toxic type 2 ribosome-inactivating proteins and are amongst the most potent toxins of plant origin.

Stirpe F., Bolognesi A., Bortolotti M., Farini V., Lubelli C., Pelosi E., et al. (2007). Characterization of highly toxic type 2 ribosome-inactivating proteins from Adenia lanceolata and Adenia stenodactyla (Passifloraceae). TOXICON, 50(1), 94-105 [10.1016/j.toxicon.2007.02.020].

Characterization of highly toxic type 2 ribosome-inactivating proteins from Adenia lanceolata and Adenia stenodactyla (Passifloraceae)

STIRPE, FIORENZO;BOLOGNESI, ANDREA;BORTOLOTTI, MASSIMO;FARINI, VALENTINA;LUBELLI, CHIARA;PELOSI, EMANUELE;POLITO, LETIZIA;DOZZA, BARBARA;STROCCHI, PAOLA;BARBIERI, LUIGI
2007

Abstract

From the caudices of the Passifloraceae Adenia lanceolata and Adenia stenodactyla, two lectins called lanceolin and stenodactylin, respectively, were purified by affinity chromatography on CL Sepharose 6B. The lectins are glycoproteins with Mr 61,243 (lanceolin) and 63,131 stenodactylin), consisting of an enzymatic A chain linked to a larger B chain with lectin properties, with N-terminal amino acid sequences similar to that of volkensin, the toxic lectin from Adenia volkensii. The lectins agglutinate red blood cells, inhibit protein synthesis both by a cell-free system and by whole cells, and depurinate ribosomes and DNA, but not tRNA or poly(A). They are highly toxic to cells, in which they induce apoptosis, and to mice, with LD50’s 8.16 μg/kg (lanceolin), and 2.76 μg/kg (stenodactylin) at 48 h. Thus lanceolin and stenodactylin have all the properties of the toxic type 2 ribosome-inactivating proteins and are amongst the most potent toxins of plant origin.
2007
Stirpe F., Bolognesi A., Bortolotti M., Farini V., Lubelli C., Pelosi E., et al. (2007). Characterization of highly toxic type 2 ribosome-inactivating proteins from Adenia lanceolata and Adenia stenodactyla (Passifloraceae). TOXICON, 50(1), 94-105 [10.1016/j.toxicon.2007.02.020].
Stirpe F.; Bolognesi A.; Bortolotti M.; Farini V.; Lubelli C.; Pelosi E.; Polito L.; Dozza B.; Strocchi P.; Chambery A.; Parente A.; Barbieri L....espandi
File in questo prodotto:
Eventuali allegati, non sono esposti

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/48342
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 40
  • ???jsp.display-item.citation.isi??? 36
social impact