A method has been developed for the analysis of the antidepressant drug sertraline together with its main metabolite N-desmethylsertraline in human plasma. It is based on capillary electrophoresis with laser-induced fluorescence (LIF) detection (lambda = 488 nm). A solid phase extraction procedure is employed for biological sample pre-treatment, followed by a derivatisation step with fluorescein isothiocyanate; reboxetine was the internal standard. The effect of cyclodextrin, acetone and N-methyl-glucamine as constituents of the background electrolyte for analyte separation was investigated. The final background electrolyte consisted of 20 mM carbonate buffer, pH 9.0, with 2.5 mM heptakis(2,6-di-O-methyl)-beta-cyclodextrin, 50 mM N-methylglucamine and 20%, v/v acetone. With 30 kV applied voltage the electrophoretic run is completed in 7.5 min. Linearity was observed in the plasma concentration range from 3.0 to 500 ng/mL for sertraline and 4.0 to 500 ng/mL for desmethylsertraline. Extraction yield was >97.1%, precision - expressed as RSD% - was <3.7, accuracy (recovery) was >95.6%. Due to its sensitivity and selectivity the method was suited for the analysis of plasma samples from patients undergoing therapy with sertraline.

A. Musenga, E. Kenndler, L. Mercolini, M. Amore, S. Fanali, M.A. Raggi (2007). Determination of sertraline and N-desmethylsertraline in human plasma by means of capillary electrophoresis with laser-induced fluorescence detection. ELECTROPHORESIS, 28(11), 1823-1831 [10.1002/elps.200600591].

Determination of sertraline and N-desmethylsertraline in human plasma by means of capillary electrophoresis with laser-induced fluorescence detection

MUSENGA, ALESSANDRO;MERCOLINI, LAURA;RAGGI, MARIA AUGUSTA
2007

Abstract

A method has been developed for the analysis of the antidepressant drug sertraline together with its main metabolite N-desmethylsertraline in human plasma. It is based on capillary electrophoresis with laser-induced fluorescence (LIF) detection (lambda = 488 nm). A solid phase extraction procedure is employed for biological sample pre-treatment, followed by a derivatisation step with fluorescein isothiocyanate; reboxetine was the internal standard. The effect of cyclodextrin, acetone and N-methyl-glucamine as constituents of the background electrolyte for analyte separation was investigated. The final background electrolyte consisted of 20 mM carbonate buffer, pH 9.0, with 2.5 mM heptakis(2,6-di-O-methyl)-beta-cyclodextrin, 50 mM N-methylglucamine and 20%, v/v acetone. With 30 kV applied voltage the electrophoretic run is completed in 7.5 min. Linearity was observed in the plasma concentration range from 3.0 to 500 ng/mL for sertraline and 4.0 to 500 ng/mL for desmethylsertraline. Extraction yield was >97.1%, precision - expressed as RSD% - was <3.7, accuracy (recovery) was >95.6%. Due to its sensitivity and selectivity the method was suited for the analysis of plasma samples from patients undergoing therapy with sertraline.
2007
A. Musenga, E. Kenndler, L. Mercolini, M. Amore, S. Fanali, M.A. Raggi (2007). Determination of sertraline and N-desmethylsertraline in human plasma by means of capillary electrophoresis with laser-induced fluorescence detection. ELECTROPHORESIS, 28(11), 1823-1831 [10.1002/elps.200600591].
A. Musenga; E. Kenndler; L. Mercolini; M. Amore; S. Fanali; M.A. Raggi
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/47481
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