Two genera of the Fabaceae family showed phytoplasma symptoms in Germany and in Italy. Spartium junceum showing typical SpaWB symptoms was observed in the city of Ercolano (Campania region, Italy) and a similar symptomatology was observed in a group of shrubs of Cytisus scoparius growing in Dahlem botanical garden in Berlin (Germany). Symptomatic and asymptomatic samples were collected in both cases and analysed for phytoplasma presence by nested-PCR assays followed by RFLP analyses. All symptomatic samples produced amplicons of the expected lengths and no product was amplified from asymptomatic plants. Identification and classification of phytoplasmas allowed to detect ‘Candidatus Phytoplasma spartii’ subgroup 16SrX-D and ‘Ca. P. asteris’ subgroup 16SrI-B in both genera. In some of the samples of C. scoparius also stolbur phytoplasmas were identified.
Contaldo, N., Paltrinieri, S., Ardizzi, S., Bertaccini, A., Bellardi, M.G., Duduk, B. (2015). Identification and Molecular Characterization of Multiple Phytoplasma Infection in Spartium Junceum and Cytisus Scoparius. ACTA HORTICULTURAE, 1072, 113-116.
Identification and Molecular Characterization of Multiple Phytoplasma Infection in Spartium Junceum and Cytisus Scoparius
CONTALDO, NICOLETTA;PALTRINIERI, SAMANTA;BERTACCINI, ASSUNTA;BELLARDI, MARIA GRAZIA;
2015
Abstract
Two genera of the Fabaceae family showed phytoplasma symptoms in Germany and in Italy. Spartium junceum showing typical SpaWB symptoms was observed in the city of Ercolano (Campania region, Italy) and a similar symptomatology was observed in a group of shrubs of Cytisus scoparius growing in Dahlem botanical garden in Berlin (Germany). Symptomatic and asymptomatic samples were collected in both cases and analysed for phytoplasma presence by nested-PCR assays followed by RFLP analyses. All symptomatic samples produced amplicons of the expected lengths and no product was amplified from asymptomatic plants. Identification and classification of phytoplasmas allowed to detect ‘Candidatus Phytoplasma spartii’ subgroup 16SrX-D and ‘Ca. P. asteris’ subgroup 16SrI-B in both genera. In some of the samples of C. scoparius also stolbur phytoplasmas were identified.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
