IL-1β and IL-8 mRNAs and proteins were quantified by Real Time RT-PCR, and western blot and related to BALF cytology in horses (n=8) during a RAO attack induced by stabling. An increase in IL-1β mRNA and protein anticipated the increase of the same parameters referred to IL-8, but related mRNA and respective protein levels were not strictly paralleled increased. The raise in IL-1β protein, but not mRNA, continued over the entire period and was correlated with the BALF neutrophilia. On the contrary, the increases of both IL-8 mRNA and protein were transient and the peak of the protein (day 8) followed the peak of BALF neutrophilia and preceded the BALF eosinophilia.
SARLI G., PELI A., TANASE O., MARROCCO R., ZANOTTI L., DUCCI A., et al. (2007). Relazione mRNA proteina per IL-1β e IL-8 nel liquido di lavaggio bronco-alveolare (BAL) di cavallo in corso di ostruzione ricorrente delle vie aeree (RAO).
Relazione mRNA proteina per IL-1β e IL-8 nel liquido di lavaggio bronco-alveolare (BAL) di cavallo in corso di ostruzione ricorrente delle vie aeree (RAO)
SARLI, GIUSEPPE;PELI, ANGELO;MARROCCO, ROMINA;BONATO, ANDREA;SASSI, FRANCESCO;BENAZZI, CINZIA;PIETRA, MARCO;CINOTTI, STEFANO
2007
Abstract
IL-1β and IL-8 mRNAs and proteins were quantified by Real Time RT-PCR, and western blot and related to BALF cytology in horses (n=8) during a RAO attack induced by stabling. An increase in IL-1β mRNA and protein anticipated the increase of the same parameters referred to IL-8, but related mRNA and respective protein levels were not strictly paralleled increased. The raise in IL-1β protein, but not mRNA, continued over the entire period and was correlated with the BALF neutrophilia. On the contrary, the increases of both IL-8 mRNA and protein were transient and the peak of the protein (day 8) followed the peak of BALF neutrophilia and preceded the BALF eosinophilia.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
