Nine Chlamydia suis isolates, obtained from pigs with conjunctivitis, were molecularly characterized by ompA sequencing and their in vitro susceptibility to six cathelicidin peptides (SMAP-29, BAC-7, BMAP-27, BMAP-27, BMAP-28, PG-1, LL-37) determined in cell culture. SMAP-29 was the most active peptide, reducing the intracellular inclusion number by ≥50% at a concentration of 10 μg/ml (3 μM) in six of the nine isolates tested. Three molecularly identical isolates were insensitive at a concentration as high as 80 μg/ml (25 μM). Of the remaining cathelicidin peptides tested, BAC-7 and BMAP-27 were active against six C. suis isolates at a concentration of 80 μg/ml (25 and 26 μM, respectively). Cathelicidins LL-37 and PG-1 did not show any anti-chlamydial activity at 80 μg/ml.
Sensitivity of Chlamydia suis to cathelicidin peptides / Donati M.; Di Francesco A.; Gennaro R.; Benincasa M.; Magnino S.; Pignanelli S.; Shurdhi A.; Moroni A.; Mazzoni C.; Merialdi G.; Baldelli R.; Cevenini R.. - In: VETERINARY MICROBIOLOGY. - ISSN 0378-1135. - STAMPA. - 123:(2007), pp. 269-273. [10.1016/j.vetmic.2007.02.011]
Sensitivity of Chlamydia suis to cathelicidin peptides.
DONATI, MANUELA;DI FRANCESCO, ANTONIETTA;PIGNANELLI, SALVATORE;SHURDHI, ALISA;BALDELLI, RAFFAELLA;CEVENINI, ROBERTO
2007
Abstract
Nine Chlamydia suis isolates, obtained from pigs with conjunctivitis, were molecularly characterized by ompA sequencing and their in vitro susceptibility to six cathelicidin peptides (SMAP-29, BAC-7, BMAP-27, BMAP-27, BMAP-28, PG-1, LL-37) determined in cell culture. SMAP-29 was the most active peptide, reducing the intracellular inclusion number by ≥50% at a concentration of 10 μg/ml (3 μM) in six of the nine isolates tested. Three molecularly identical isolates were insensitive at a concentration as high as 80 μg/ml (25 μM). Of the remaining cathelicidin peptides tested, BAC-7 and BMAP-27 were active against six C. suis isolates at a concentration of 80 μg/ml (25 and 26 μM, respectively). Cathelicidins LL-37 and PG-1 did not show any anti-chlamydial activity at 80 μg/ml.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
