Collective migration is an important cellular trait, which is intensely studied by both basic and translational researchers. Investigation of the underlying mechanisms necessitates high-throughput assays and computational algorithms capable of generating reproducible quantitative measurements of cell migration. We present a desktop tool that can be used easily by any researcher, to quantify both fluorescent and phase-contrast images produced in the course of commonly used gap closure ("scratch," "wound healing") collective migration assays. The software has a simple graphical interface that allows the user to tune the relevant parameters and process large numbers of images (or movies). The output contains segmented images and the numerical values inferred from them, allowing easy quantitative analysis of the results.
qCMA: A Desktop Application for Quantitative Collective Cell Migration Analysis / A. Zeisel; A. Yitzhaky; C. Koerner; M. Lauriola; H. Cohen-Dvashi; W. J. Kostler; Y. Yarden; S. Wiemann; E. Domany. - In: JOURNAL OF BIOMOLECULAR SCREENING. - ISSN 1087-0571. - STAMPA. - 18:3(2013), pp. 356-360. [10.1177/1087057112461940]
qCMA: A Desktop Application for Quantitative Collective Cell Migration Analysis
LAURIOLA, MATTIA;
2013
Abstract
Collective migration is an important cellular trait, which is intensely studied by both basic and translational researchers. Investigation of the underlying mechanisms necessitates high-throughput assays and computational algorithms capable of generating reproducible quantitative measurements of cell migration. We present a desktop tool that can be used easily by any researcher, to quantify both fluorescent and phase-contrast images produced in the course of commonly used gap closure ("scratch," "wound healing") collective migration assays. The software has a simple graphical interface that allows the user to tune the relevant parameters and process large numbers of images (or movies). The output contains segmented images and the numerical values inferred from them, allowing easy quantitative analysis of the results.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
