Until now, significant differences in the neurochemical pattern of enteric neurons have been demonstrated in all species studied; however, some strong similarities also occur across species, such as the occurrence of nitric oxide synthase immunoreactivity (NOS-IR) in inhibitory motor neurons to muscle. In consideration of the insufficient data regarding the enteric nervous system (ENS) of sheep, we investigated the myenteric plexus and submucosal plexus of the ovine ileum. Since the pivotal role of the ENS in the early pathogenesis of sheep scrapie, the “prototype” of prion diseases, has been suggested, we have focused our observations also on the host’s PrP genotype. We have studied the morphology and distribution of NOS-IR neurons and their relationships with the enteric glia in whole-mount preparations and in cryostat sections. NOS-IR neurons, always encircled by glial processes, were located in both plexuses. Many NOS-IR fibers were seen in the circular muscle layer, in the submucosa, and in the mucosa. In the submucosa they were close to the lymphoid tissue. No differences in the distribution and percentage of NOS-IR fibers and neurons were observed among sheep carrying different PrP genotype, thus making unlikely their contribution in the determinism of susceptibility/resistance to scrapie infection.

NOS-immunoreactivity and NADPH-d histochemistry in the enteric nervous system of Sarda breed sheep with different PrP genotypes in whole-mount and cryostat preparations / LALATTA COSTERBOSA G.; MAZZONI M.; CLAVENZANI P.; DI GUARDO G.; MAZZUOLI G.; MARRUCHELLA G.; DE GROSSI L.; AGRIMI U.; CHIOCCHETTI R.. - In: JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY. - ISSN 0022-1554. - STAMPA. - 55/4:(2007), pp. 387-401. [10.1369/jhc.6A7052.2007]

NOS-immunoreactivity and NADPH-d histochemistry in the enteric nervous system of Sarda breed sheep with different PrP genotypes in whole-mount and cryostat preparations.

LALATTA COSTERBOSA, GIOVANNA;MAZZONI, MAURIZIO;CLAVENZANI, PAOLO;MAZZUOLI, GEMMA;CHIOCCHETTI, ROBERTO
2007

Abstract

Until now, significant differences in the neurochemical pattern of enteric neurons have been demonstrated in all species studied; however, some strong similarities also occur across species, such as the occurrence of nitric oxide synthase immunoreactivity (NOS-IR) in inhibitory motor neurons to muscle. In consideration of the insufficient data regarding the enteric nervous system (ENS) of sheep, we investigated the myenteric plexus and submucosal plexus of the ovine ileum. Since the pivotal role of the ENS in the early pathogenesis of sheep scrapie, the “prototype” of prion diseases, has been suggested, we have focused our observations also on the host’s PrP genotype. We have studied the morphology and distribution of NOS-IR neurons and their relationships with the enteric glia in whole-mount preparations and in cryostat sections. NOS-IR neurons, always encircled by glial processes, were located in both plexuses. Many NOS-IR fibers were seen in the circular muscle layer, in the submucosa, and in the mucosa. In the submucosa they were close to the lymphoid tissue. No differences in the distribution and percentage of NOS-IR fibers and neurons were observed among sheep carrying different PrP genotype, thus making unlikely their contribution in the determinism of susceptibility/resistance to scrapie infection.
2007
NOS-immunoreactivity and NADPH-d histochemistry in the enteric nervous system of Sarda breed sheep with different PrP genotypes in whole-mount and cryostat preparations / LALATTA COSTERBOSA G.; MAZZONI M.; CLAVENZANI P.; DI GUARDO G.; MAZZUOLI G.; MARRUCHELLA G.; DE GROSSI L.; AGRIMI U.; CHIOCCHETTI R.. - In: JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY. - ISSN 0022-1554. - STAMPA. - 55/4:(2007), pp. 387-401. [10.1369/jhc.6A7052.2007]
LALATTA COSTERBOSA G.; MAZZONI M.; CLAVENZANI P.; DI GUARDO G.; MAZZUOLI G.; MARRUCHELLA G.; DE GROSSI L.; AGRIMI U.; CHIOCCHETTI R.
File in questo prodotto:
Eventuali allegati, non sono esposti

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/46446
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? 6
  • Scopus 20
  • ???jsp.display-item.citation.isi??? 18
social impact