Several studies have been recently focused on the production of specialty chemicals such as flavour compounds from wastes or surplus of the agro-industry as a possible way of both disposing and valorising them. Cereal bran, one of the most widely produced agricultural processing by-product, can be of interest in vanillin production as it is a potential source of ferulic acid, from which vanillin can be obtained via microbial bioconversion. Ferulic acid is linked to cell wall polysaccharides through ester links, which have to be hydrolyzed to release ferulic acid. The aim of this work was to produce vanillin from wheat bran through: a) enzymatic hydrolysis of wheat bran and b) bioconversion of the resulting ferulic acid into vanillin by employing a recombinant E. coli strain containing the genes for the bioconversion of ferulic acid to vanillin (E. coli JM109/pBB1). Wheat bran was hydrolyzed with a mixture of two commercially available enzymes, thus obtaining 1323 mg of ferulic acid/kg of dried wheat bran. The bran hydrolyzate also contained a high carbohydrate concentration. Bioconversion of ferulic acid present in the extract was assayed by using resting cells of E. coli JM109/pBB1, thus obtaining a vanillin molar yield of 50% from an initial ferulic acid concentration of 0.75 mM. However, this yield was significantly lower than that obtained with pure ferulic acid at the same initial concentration. Therefore, ferulic acid was recovered from the crude bran extracts by employing ion exchange resins with the concomitant removal of carbohydrates. In these conditions, vanillin molar yield was increased up to 70%. The results obtained pointed out that vanillin can be obtained at interesting yields from wheat bran, an agriculture by-product produced in large amounts in Italy.

Vanillin production from ferulic acid obtained from wheat bran / D. Di Gioia; L. Sciubba; L. Setti; F. Luziatelli; M. Ruzzi; F. Fava. - STAMPA. - (2007), pp. Pag. 271-274. (Intervento presentato al convegno Italic4 tenutosi a Monte Porzio Catone, Rome, Italy nel May 8-10, 2007).

Vanillin production from ferulic acid obtained from wheat bran.

DI GIOIA, DIANA;SCIUBBA, LUIGI;SETTI, LEONARDO;FAVA, FABIO
2007

Abstract

Several studies have been recently focused on the production of specialty chemicals such as flavour compounds from wastes or surplus of the agro-industry as a possible way of both disposing and valorising them. Cereal bran, one of the most widely produced agricultural processing by-product, can be of interest in vanillin production as it is a potential source of ferulic acid, from which vanillin can be obtained via microbial bioconversion. Ferulic acid is linked to cell wall polysaccharides through ester links, which have to be hydrolyzed to release ferulic acid. The aim of this work was to produce vanillin from wheat bran through: a) enzymatic hydrolysis of wheat bran and b) bioconversion of the resulting ferulic acid into vanillin by employing a recombinant E. coli strain containing the genes for the bioconversion of ferulic acid to vanillin (E. coli JM109/pBB1). Wheat bran was hydrolyzed with a mixture of two commercially available enzymes, thus obtaining 1323 mg of ferulic acid/kg of dried wheat bran. The bran hydrolyzate also contained a high carbohydrate concentration. Bioconversion of ferulic acid present in the extract was assayed by using resting cells of E. coli JM109/pBB1, thus obtaining a vanillin molar yield of 50% from an initial ferulic acid concentration of 0.75 mM. However, this yield was significantly lower than that obtained with pure ferulic acid at the same initial concentration. Therefore, ferulic acid was recovered from the crude bran extracts by employing ion exchange resins with the concomitant removal of carbohydrates. In these conditions, vanillin molar yield was increased up to 70%. The results obtained pointed out that vanillin can be obtained at interesting yields from wheat bran, an agriculture by-product produced in large amounts in Italy.
2007
Science and Technology of Biomasses: Advances and Challenges. Materials, Chemicals and Processes from Agricultural and Forest Biomass. Proceedings book
Pag. 271
274
Vanillin production from ferulic acid obtained from wheat bran / D. Di Gioia; L. Sciubba; L. Setti; F. Luziatelli; M. Ruzzi; F. Fava. - STAMPA. - (2007), pp. Pag. 271-274. (Intervento presentato al convegno Italic4 tenutosi a Monte Porzio Catone, Rome, Italy nel May 8-10, 2007).
D. Di Gioia; L. Sciubba; L. Setti; F. Luziatelli; M. Ruzzi; F. Fava
File in questo prodotto:
Eventuali allegati, non sono esposti

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/45403
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact