Direct inkjet printing of a complete and working amperometric biosensor for the detection of hydrogen peroxide, based on horseradish peroxidase (HRP), has been demonstrated. The device has been realized with a commercial printer. A thin layer of PEDOT:PSS, which was in turn covered with HRP, was inkjet printed on top of an ITO-coated glass slide. The active components of the device retained their properties after the thermal inkjet printing. The whole device has been encapsulated by means of a selectively permeable cellulose acetate membrane. The successful electron transfer between the PEDOT:PSS covered electrode and the enzyme has been demonstrated, and the biosensor evidenced very good sensitivity, in line with the best devices realized with other techniques, and a remarkable operational stability. This result paves the way for an extensive application of “biopolytronics”, i.e. the utilization of conductive/semiconductive polymers and biologically active molecules to design bioelectronic devices using a common PC, and exploiting normal commercial printers to print them out.

An HRP-based amperometric biosensor fabricated by thermal inkjet printing / L. Setti; A. Fraleoni-Morgera; I. Mencarelli; A. Filippini; B. Ballarin; M. Di Biase. - In: SENSORS AND ACTUATORS. B, CHEMICAL. - ISSN 0925-4005. - STAMPA. - 126:(2007), pp. 252-257. [10.1016/j.snb.2006.12.015]

An HRP-based amperometric biosensor fabricated by thermal inkjet printing

SETTI, LEONARDO;BALLARIN, BARBARA;
2007

Abstract

Direct inkjet printing of a complete and working amperometric biosensor for the detection of hydrogen peroxide, based on horseradish peroxidase (HRP), has been demonstrated. The device has been realized with a commercial printer. A thin layer of PEDOT:PSS, which was in turn covered with HRP, was inkjet printed on top of an ITO-coated glass slide. The active components of the device retained their properties after the thermal inkjet printing. The whole device has been encapsulated by means of a selectively permeable cellulose acetate membrane. The successful electron transfer between the PEDOT:PSS covered electrode and the enzyme has been demonstrated, and the biosensor evidenced very good sensitivity, in line with the best devices realized with other techniques, and a remarkable operational stability. This result paves the way for an extensive application of “biopolytronics”, i.e. the utilization of conductive/semiconductive polymers and biologically active molecules to design bioelectronic devices using a common PC, and exploiting normal commercial printers to print them out.
2007
An HRP-based amperometric biosensor fabricated by thermal inkjet printing / L. Setti; A. Fraleoni-Morgera; I. Mencarelli; A. Filippini; B. Ballarin; M. Di Biase. - In: SENSORS AND ACTUATORS. B, CHEMICAL. - ISSN 0925-4005. - STAMPA. - 126:(2007), pp. 252-257. [10.1016/j.snb.2006.12.015]
L. Setti; A. Fraleoni-Morgera; I. Mencarelli; A. Filippini; B. Ballarin; M. Di Biase
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/44818
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