A method for the detection of Listeria monocytogenes in food in two days is presented. The method consists of a two-step enrichment, bacterial cells lysis and a closed-tube duplex 5'-nuclease PCR with an internal amplification control, which is read in end-point mode in a 96-well fluorimeter. The 5'-nuclease PCR has an intrinsic detection limit of 104 CFU ml-1 and facilitates monitoring of false negative results caused by the failure of amplification. The complete method for the detection of L. monocytogenes in food has a detection limit of 100 CFU (10 g)-1 and does not produce false positive results due to the presence of dead cells.
K.Oravcová, E.Kaclíková, P.Siekel, S.Girotti, T.Kuchta. (2007). Detection of Listeria monocytogenes in food in two days using enrichment and 5' nuclease polymerase chain reaction with end-point fluorimetry. JOURNAL OF FOOD AND NUTRITION RESEARCH, 46, 35-38.
Detection of Listeria monocytogenes in food in two days using enrichment and 5' nuclease polymerase chain reaction with end-point fluorimetry
GIROTTI, STEFANO;
2007
Abstract
A method for the detection of Listeria monocytogenes in food in two days is presented. The method consists of a two-step enrichment, bacterial cells lysis and a closed-tube duplex 5'-nuclease PCR with an internal amplification control, which is read in end-point mode in a 96-well fluorimeter. The 5'-nuclease PCR has an intrinsic detection limit of 104 CFU ml-1 and facilitates monitoring of false negative results caused by the failure of amplification. The complete method for the detection of L. monocytogenes in food has a detection limit of 100 CFU (10 g)-1 and does not produce false positive results due to the presence of dead cells.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
