In this study we screened 48 gastrointestinal carcinomas, 13 urogenital and 7 oral carcinomas in parallel with control samples for the 15 STR loci of the AmpFlSTR Identifiler Kit to provide further data useful to evaluate the applicability on cancerous tissues of STRs used in forensic field. A total of 37 cancerous tissues (54.4%) showed allelic alterations when compared with the corresponding normal tissues: 29 (78.4%) gastrointestinal tumors, 4 (10.8%) urogenital tumors and 4 (10.8%) oral tumors. The loci most frequently affected by allelic alterations were VWA, FGA and D18S51. These results suggest that great care should be taken in the evaluation of the DNA typing results obtained from clinical cancerous specimens when no other reference samples containing normal tissue are available.
Evaluation of reliability of STR typing in different types of cancerous tissues used for identification purpose / S. Ceccardi; M. Alù; F. Lugaresi; G. Ferri; C. Bini; T. Balbi; F. Ingravallo; S. Pelotti.. - STAMPA. - (2006), pp. 672-675. [10.1016/j.ics.2005.11.044]
Evaluation of reliability of STR typing in different types of cancerous tissues used for identification purpose.
CECCARDI, STEFANIA;LUGARESI, FEDERICA;FERRI, GIANMARCO;BINI, CARLA;BALBI, TIZIANA;INGRAVALLO, FRANCESCA;PELOTTI, SUSI
2006
Abstract
In this study we screened 48 gastrointestinal carcinomas, 13 urogenital and 7 oral carcinomas in parallel with control samples for the 15 STR loci of the AmpFlSTR Identifiler Kit to provide further data useful to evaluate the applicability on cancerous tissues of STRs used in forensic field. A total of 37 cancerous tissues (54.4%) showed allelic alterations when compared with the corresponding normal tissues: 29 (78.4%) gastrointestinal tumors, 4 (10.8%) urogenital tumors and 4 (10.8%) oral tumors. The loci most frequently affected by allelic alterations were VWA, FGA and D18S51. These results suggest that great care should be taken in the evaluation of the DNA typing results obtained from clinical cancerous specimens when no other reference samples containing normal tissue are available.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
