The alpha-hydroxyacid 2-hydroxy-4-methylthiobutanoic acid (the so-called methionine hydroxy-analogue, MHA), largely used in animal nutrition as a source of methionine, forms stable metal chelates with divalent metals of formula [{CH3SCH2CH2CH(OH)COO}2M].nH2O. Protonation and iron(III) and copper(II) complex formation constants have been determined by pH-metry at 25 °C. In vivo investigations were carried out in the rat. After receiving a zinc-deficient diet for 3 weeks, animals were fed the same diet added with zinc sulfate or zinc/MHA chelate; the zinc content of faeces was higher (+ 45%; P < 0.05) in sulfate fed rats, whereas zinc retention was higher (+ 61%; P < 0.05) in the Zn/MHA diet. Moreover, experiments in vitro with human intestinal Caco-2 cells indicated that the MHA/Fe chelate was taken up by the cells without any apparent toxic effect.

Metal chelates of 2-hydroxy-4-methylthiobutanoic acid in animal feeding. Part 2: further characterizations, in vitro and in vivo investigations

BIAGI, GIACOMO;
2005

Abstract

The alpha-hydroxyacid 2-hydroxy-4-methylthiobutanoic acid (the so-called methionine hydroxy-analogue, MHA), largely used in animal nutrition as a source of methionine, forms stable metal chelates with divalent metals of formula [{CH3SCH2CH2CH(OH)COO}2M].nH2O. Protonation and iron(III) and copper(II) complex formation constants have been determined by pH-metry at 25 °C. In vivo investigations were carried out in the rat. After receiving a zinc-deficient diet for 3 weeks, animals were fed the same diet added with zinc sulfate or zinc/MHA chelate; the zinc content of faeces was higher (+ 45%; P < 0.05) in sulfate fed rats, whereas zinc retention was higher (+ 61%; P < 0.05) in the Zn/MHA diet. Moreover, experiments in vitro with human intestinal Caco-2 cells indicated that the MHA/Fe chelate was taken up by the cells without any apparent toxic effect.
Predieri G.; Elviri L.; Tegoni M.; Zagnoni I.; Cinti E.; Biagi G.; Ferruzzi S.; Leonardi G.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11585/4323
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