Uneven illumination affects every image acquired by a microscope. It is often overlooked, but it can introduce considerable bias to image measurements. The most reliable correction methods require special reference images, and retrospective alternatives do not fully model the correction process. Our approach overcomes these issues for most optical microscopy applications without the need for reference images.
Kevin Smith, Yunpeng Li, Filippo Piccinini, Gábor Csúcs, Csaba Balazs, Alessandro Bevilacqua, et al. (2015). CIDRE: an illumination-correction method for optical microscopy. NATURE METHODS, 12(5), 404-406 [10.1038/nmeth.3323].
CIDRE: an illumination-correction method for optical microscopy
PICCININI, FILIPPO;BEVILACQUA, ALESSANDRO;
2015
Abstract
Uneven illumination affects every image acquired by a microscope. It is often overlooked, but it can introduce considerable bias to image measurements. The most reliable correction methods require special reference images, and retrospective alternatives do not fully model the correction process. Our approach overcomes these issues for most optical microscopy applications without the need for reference images.| File | Dimensione | Formato | |
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